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Immunohistochemical vs Molecular Biology Methods:Complementary Techniques for Effective Screening of p53 Alterations in Head and Neck Cancer
- Source :
- American Journal of Clinical Pathology. 107:7-11
- Publication Year :
- 1997
- Publisher :
- Oxford University Press (OUP), 1997.
-
Abstract
- The purpose of this study was to correlate p53 gene alterations and their expression in 85 head and neck squamous cell carcinomas. Genomic p53 was amplified with the polymerase chain reaction (PCR) from formalin-fixed, paraffin-embedded tissues. Exons 5 through 8 were screened for mutations by means of non-isotopic single-strand conformation polymorphism (SSCP) analysis. p53 expression was detected by means of immunohistochemistry (IHC) with the p53 monoclonal antibody DO-7. Twenty-three lesions (27%) showed both SSCP variants and DO-7 immunostaining, whereas 37 (44%) demonstrated both SSCP normal patterns and negative staining. Twenty-five lesions (29%) were discordant, including 12 IHC-positive (14%) and 13 SSCP-positive (15%) lesions. However, discordant IHC-negative, SSCP-positive lesions could have been easily interpreted after sequencing of the abnormal samples. Had these been screened with IHC only, all nonsense or frameshift p53 mutations would have been missed. IHC-positive, SSCP-negative lesions were interpreted in light of current models of p53 immunodetection. Had these been screened with SSCP or sequencing only, a sizeable number of tumors expressing p53 protein abnormalities would have been undetected. Therefore, simultaneous use of both methods increases the number of p53 abnormalities detected, and is warranted.
- Subjects :
- Pathology
medicine.medical_specialty
Gene Expression
Biology
Polymerase Chain Reaction
Frameshift mutation
law.invention
Immunoenzyme Techniques
Exon
law
medicine
Humans
Gene
Polymorphism, Single-Stranded Conformational
Polymerase chain reaction
Antibodies, Monoclonal
Single-strand conformation polymorphism
DNA, Neoplasm
General Medicine
Genes, p53
Epidermoid carcinoma
Head and Neck Neoplasms
Carcinoma, Squamous Cell
Immunohistochemistry
Tumor Suppressor Protein p53
Immunostaining
Subjects
Details
- ISSN :
- 19437722 and 00029173
- Volume :
- 107
- Database :
- OpenAIRE
- Journal :
- American Journal of Clinical Pathology
- Accession number :
- edsair.doi.dedup.....7df56fcc266cb24deab6d4b266fc9c60