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Looking beyond endotoxin: a comparative study of pyrogen retention by ultrafilters used for the preparation of sterile dialyis fluid
- Source :
- SCIENTIFIC REPORTS, Scientific Reports
- Publication Year :
- 2014
- Publisher :
- Nature Publishing Group, 2014.
-
Abstract
- Sterile single-use ultrafilters are used in dialysis for the preparation of the substitution fluid given to patients undergoing dialysis treatments with high convective fluid removal. The retention of pyrogenic agents by the ultrafilters is crucial to avoiding inflammatory responses. The performance of a new single-use ultrafilter (NUF) with a positively charged flat sheet membrane of relatively small membrane area and large pore size was compared to a reference ultrafilter (RUF) with a hollow fiber membrane. Filter performance was tested with various pyrogen-contaminated dialysis fluids by direct pyrogen quantification and by measuring inflammatory responses in cell-based bioassays. The NUF completely retained oligodeoxynucleotides (ODN), whereas the RUF was fully permeable. Both filters tended to decrease biological activity of DNA in filtered bacterial lysates. The NUF reduced lipopolysaccharides (LPS) and LPS-induced biological activity by 100%, whereas the RUF produced filtrates with low but detectable levels of LPS in most cases. Peptidoglycans (PGN) were fully retained both by the NUF and the RUF. The new ultrafilter retained biologically active ODN, which has not yet been described for any other device used in dialysis and it showed better or equal retention of LPS and PGN even with a smaller membrane surface and larger pore size.
- Subjects :
- Lipopolysaccharides
Pore size
Flat sheet membrane
Pathology
medicine.medical_specialty
BACTERIAL-DNA
Ultrafiltration
HEMODIALYSIS-PATIENTS
Article
Mice
REMOVAL
Renal Dialysis
Medicine and Health Sciences
medicine
Animals
Humans
Bioassay
FILTERS
CARDIOVASCULAR EVENTS
Membrane surface
ALL-CAUSE MORTALITY
Multidisciplinary
Chromatography
Pyrogens
Chemistry
Membranes, Artificial
Biological activity
Hemodialysis Solutions
Endotoxins
Membrane
Hollow fiber membrane
Leukocytes, Mononuclear
NIH 3T3 Cells
Dialysis (biochemistry)
ONLINE HEMODIAFILTRATION
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....7def69d91477020a0a781aa5c3440231
- Full Text :
- https://doi.org/10.1038/srep06390