Back to Search
Start Over
Average Conformation of Branched Chain Lipid PGP-Me That Accounts for the Thermal Stability and High-Salinity Resistance of Archaeal Membranes
- Source :
- Biochemistry. 58:3869-3879
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- The average conformation of the methyl-branched chains of archaeal lipid phosphatidyl glycerophosphate methyl ester (PGP-Me) was examined in a hydrated bilayer membrane based on the 2H nuclear magnetic resonance (NMR) of enantioselectively 2H-labeled compounds that were totally synthesized for the first time in this study. The NMR results in combination with molecular dynamics simulations revealed that the PGP-Me chain appeared to exhibit behavior different from that of typical membrane lipids such as dimyristoylphosphatidylcholine (DMPC). The C-C bonds of the PGP-Me chain adopt alternative parallel and tilted orientations to the membrane normal as opposed to a DMPC chain where all of the C-C bonds tilt in the same way on average. This characteristic orientation causes the intertwining of PGP-Me chains, which plays an important role in the excellent thermal and high-salinity stabilities of archaeal lipid bilayers and membrane proteins.
- Subjects :
- Salinity
0303 health sciences
Hot Temperature
Magnetic Resonance Spectroscopy
Chemistry
Membrane lipids
Bilayer
030302 biochemistry & molecular biology
Molecular Dynamics Simulation
Archaea
Biochemistry
03 medical and health sciences
Crystallography
Molecular dynamics
Membrane
Purple Membrane
Chain (algebraic topology)
Membrane protein
polycyclic compounds
lipids (amino acids, peptides, and proteins)
Thermal stability
Lipid bilayer
Phospholipids
Subjects
Details
- ISSN :
- 15204995 and 00062960
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Biochemistry
- Accession number :
- edsair.doi.dedup.....7dde979ae08a44877233506425cd289d