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Expression of Cyclooxygenase-1 and -2 in Human Breast Cancer
- Source :
- Surgery Today. 33:805-811
- Publication Year :
- 2003
- Publisher :
- Springer Science and Business Media LLC, 2003.
-
Abstract
- Purpose. We investigated the expression of cyclooxygenase (Cox-1 and Cox-2) in mammary tissues from patients with breast cancer. Methods. We used reverse transcriptase–polymerase chain reaction (RT-PCR) and immunohistochemistry. Results. The cancer cells showed very weak expression of Cox-1, but strong expression of immunoreactive Cox-2. In contrast, immunoreactive Cox-2 was very weak in all of the benign mammary tumors examined, including fibroadenoma (FA) and mastopathy (MP). Immunoreactive Cox-1 was also very weak in these benign tumors. The extent and intensity of immunoreactive Cox-2 polypeptides was significantly greater in the cancer cells than in the FA cells or MP cells. RT-PCR analysis showed enhanced expression of Cox-2, but not Cox-1 in breast cancer tissue, and faint expression of Cox-2 in benign tissue. Conclusions. These results demonstrated that human breast cancer cells generated Cox-2, indicating that Cox-2 might play an important role in the proliferation of breast cancer cells.
- Subjects :
- Adult
Pathology
medicine.medical_specialty
Breast Neoplasms
Sensitivity and Specificity
Statistics, Nonparametric
Cohort Studies
Breast cancer
Surgical oncology
Culture Techniques
Biomarkers, Tumor
medicine
Humans
Aged
Neoplasm Staging
biology
Reverse Transcriptase Polymerase Chain Reaction
business.industry
Biopsy, Needle
Membrane Proteins
Cancer
General Medicine
Middle Aged
Prognosis
medicine.disease
Immunohistochemistry
Fibroadenoma
Isoenzymes
Cyclooxygenase 2
Prostaglandin-Endoperoxide Synthases
Cancer cell
Cyclooxygenase 1
biology.protein
Female
Surgery
Cyclooxygenase
business
Human breast
Subjects
Details
- ISSN :
- 14362813 and 09411291
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Surgery Today
- Accession number :
- edsair.doi.dedup.....7dd6afdfb06bd320c6e0689a1bf1ce93