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The expression and methylation status of vitamin D receptor gene in Behcet's disease
- Source :
- Immunity, Inflammation and Disease, Immunity, Inflammation and Disease, Vol 7, Iss 4, Pp 308-317 (2019)
- Publication Year :
- 2019
-
Abstract
- Introduction Vitamin D has important roles as a natural immune modulator via regulating the expression of genes which have been implicated in the pathophysiology of autoimmune diseases. Vitamin D function and its deficiency have been linked to a wide range of metabolic disorders including disorders of calcium metabolism, malignant, cardiovascular, infectious, neuromuscular, and inflammatory diseases. Environmental factors, genetic factors, and epigenetic changes contribute to Behcet's disease (BD) development. The aim of our study was to analyze the expression level and methylation status of the vitamin D receptor (VDR) gene promoter in the peripheral blood mononuclear cells (PBMCs) of patients with BD. Methods In a case‐control study, 48 Iranian Azeri patients with BD and 60 age‐, sex‐ and ethnically‐matched healthy controls were included. Venous blood samples were collected and PBMCs were isolated by Ficoll protocol. The DNA and RNA were subsequently extracted. Promoter methylation levels were evaluated by MeDIP‐quantitative polymerase chain reaction (qPCR). The expression of VDR was evaluated by real‐time PCR. Results The results of quantitative real‐time PCR analysis showed that the level of VDR expression in patients with BD was significantly lower than the control group (P = .013). There was no significant difference in the level of DNA methylation in the BD and control groups (P > .05). As the results show, the expression level of VDR gene was significantly different between female and male in the patient group (P = .001). VDR gene expression was significantly higher in subjects with phlebitis. No correlation was observed between VDR gene expression rate and BD activity. Conclusion VDR gene expression decreased in patients with BD. However, there is no suggestion evidence that the expression level of VDR is regulated by a unique DNA methylation mechanism. No correlation exists between VDR gene expression and BD activity.
- Subjects :
- 0301 basic medicine
lcsh:Immunologic diseases. Allergy
Adult
Male
medicine.medical_specialty
Immunology
Calcitriol receptor
Epigenesis, Genetic
03 medical and health sciences
0302 clinical medicine
Internal medicine
Gene expression
Vitamin D and neurology
medicine
Immunology and Allergy
Humans
Epigenetics
Gene
Original Research
business.industry
Behcet Syndrome
Promoter
Methylation
DNA Methylation
Middle Aged
Behcet's disease
030104 developmental biology
Endocrinology
Case-Control Studies
vitamin D receptor (VDR)
DNA methylation
gene expression
Receptors, Calcitriol
Female
methylation
business
lcsh:RC581-607
epigenetic
030215 immunology
Subjects
Details
- ISSN :
- 20504527
- Volume :
- 7
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Immunity, inflammation and disease
- Accession number :
- edsair.doi.dedup.....7db94ee24da7f6c9a2aa958dddc341cf