Back to Search
Start Over
Differential sialylation of serpin A1 in the early diagnosis of Parkinson's disease dementia
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 11, p e48783 (2012)
- Publication Year :
- 2012
-
Abstract
- The prevalence of Parkinson’s disease (PD) increases with age. Up to 50% of PD show cognitive decline in terms of a mild cognitive impairment already in early stages that predict the development of dementia, which can occur in up to 80% of PD patients over the long term, called Parkinson’s disease dementia (PDD). So far, diagnosis of PD/PDD is made according to clinical and neuropsychological examinations while laboratory data is only used for exclusion of other diseases. The aim of this study was the identification of possible biomarkers in cerebrospinal fluid (CSF) of PD, PDD and controls (CON) which predict the development of dementia in PD. For this, a proteomic approach optimized for CSF was performed using 18 clinically well characterized patients in a first step with subsequent validation using 84 patients. Here, we detected differentially sialylated isoforms of Serpin A1 as marker for differentiation of PD versus PDD in CSF. Performing 2Dimmunoblots, all PDD patients could be identified correctly (sensitivity 100%). Ten out of 24 PD patients showed Serpin A1 isoforms in a similar pattern like PDD, indicating a specificity of 58% for the test-procedure. In control samples, no additional isoform was detected. On the basis of these results, we conclude that differentially sialylated products of Serpin A1 are an interesting biomarker to indicate the development of a dementia during the course of PD. peerReviewed
- Subjects :
- Proteomics
Oncology
Parkinson's disease
Proteome
Medizin
Disease
Biochemistry
0302 clinical medicine
Cerebrospinal fluid
Pathology
Protein Isoforms
Cognitive decline
Psychiatry
0303 health sciences
Spectrometric Identification of Proteins
Multidisciplinary
Proteomic Databases
Brain
Parkinson Disease
Neurodegenerative Diseases
Clinical Laboratory Sciences
3. Good health
Mental Health
Neurology
Medicine
Biomarker (medicine)
Alzheimer's disease
Sequence Analysis
Research Article
medicine.medical_specialty
animal structures
Clinical Research Design
Differential Sialylation
Serpin A1
Diagnosis
Parkinson’s Disease
Dementia
Science
Sensitivity and Specificity
03 medical and health sciences
Diagnostic Medicine
Internal medicine
mental disorders
medicine
Humans
Biology
030304 developmental biology
Dementia with Lewy bodies
business.industry
Proteins
medicine.disease
alpha 1-Antitrypsin
Immunology
business
Protein Processing, Post-Translational
Biomarkers
030217 neurology & neurosurgery
General Pathology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- PLoS ONE, PLoS ONE, Vol 7, Iss 11, p e48783 (2012)
- Accession number :
- edsair.doi.dedup.....7da5a34b61160fcaa936ba7bef4870a0