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Flow cytometry for fast screening and automated risk assessment in systemic light-chain amyloidosis
- Source :
- LEUKEMIA, r-FISABIO. Repositorio Institucional de Producción Científica, instname, r-FISABIO: Repositorio Institucional de Producción Científica, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO), Leukemia, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, Dadun. Depósito Académico Digital de la Universidad de Navarra, Digital.CSIC. Repositorio Institucional del CSIC
- Publication Year :
- 2019
- Publisher :
- NATURE PUBLISHING GROUP, 2019.
-
Abstract
- Early diagnosis and risk stratification are key to improve outcomes in light-chain (AL) amyloidosis. Here we used multidimensional-flow-cytometry (MFC) to characterize bone marrow (BM) plasma cells (PCs) from a series of 166 patients including newly-diagnosed AL amyloidosis (N = 94), MGUS (N = 20) and multiple myeloma (MM, N = 52) vs. healthy adults (N = 30). MFC detected clonality in virtually all AL amyloidosis (99%) patients. Furthermore, we developed an automated risk-stratification system based on BMPCs features, with independent prognostic impact on progression-free and overall survival of AL amyloidosis patients (hazard ratio: ≥ 2.9;P ≤.03). Simultaneous assessment of the clonal PCs immunophenotypic protein expression profile and the BM cellular composition, mapped AL amyloidosis in the crossroad between MGUS and MM; however, lack of homogenously-positive CD56 expression, reduction of B-cell precursors and a predominantly-clonal PC compartment in the absence of an MM-like tumor PC expansion, emerged as hallmarks of AL amyloidosis (ROC-AUC = 0.74;P<br />This study was supported by the Centro de Investigación Biomédica en Red – Área de Oncología—del Instituto de Salud Carlos III (CIBERONC; CB16/12/00369, CB16/12/00400 and CB16/12/00489), Instituto de Salud Carlos III/Subdirección General de Investigación Sanitaria (FIS No. PI13/02196), Asociación Española Contra el Cáncer (GCB120981SAN and Accelerator Award), the Black Swan Research Initiative of the International Myeloma Foundation, and the European Research Council (ERC) 2015 Starting Grant (MYELOMANEXT).
- Subjects :
- Male
0301 basic medicine
Oncology
Cancer Research
Identification
Plasma-cells
Myeloma
Translocation (11/14)
0302 clinical medicine
Immunophenotyping
Bone Marrow
Mass Screening
Immunoglobulin Light-chain Amyloidosis
Multiple myeloma
Aged, 80 and over
Amyloidosis
Diagnosed AL amyloidosis
Hematology
Middle Aged
Flow Cytometry
Immunoglobulin Isotypes
medicine.anatomical_structure
030220 oncology & carcinogenesis
CD43 expression
Female
Adult
medicine.medical_specialty
Risk Assessment
Differential-diagnosis
Clonal Evolution
Multiple-myeloma
03 medical and health sciences
Internal medicine
medicine
AL amyloidosis
Humans
Adverse prognostic-factor
Mass screening
Aged
Neoplasm Staging
Haematological cancer
business.industry
Minimal residual disease
Translational research
medicine.disease
Staging system
030104 developmental biology
Bone marrow
Differential diagnosis
business
Biomarkers
Subjects
Details
- ISSN :
- 08876924
- Database :
- OpenAIRE
- Journal :
- LEUKEMIA, r-FISABIO. Repositorio Institucional de Producción Científica, instname, r-FISABIO: Repositorio Institucional de Producción Científica, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO), Leukemia, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, Dadun. Depósito Académico Digital de la Universidad de Navarra, Digital.CSIC. Repositorio Institucional del CSIC
- Accession number :
- edsair.doi.dedup.....7d8d6eb0f7ba84584ea84ecc532b4e9e