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Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes
- Source :
- Proceedings of the National Academy of Sciences of the United States of America. 85(12)
- Publication Year :
- 1988
-
Abstract
- Two cDNA clones were isolated from a human liver library that encode two phosphatase 2A catalytic subunits. The two cDNAs differed in eight amino acids (97% identity) with three nonconservative substitutions. All of the amino acid substitutions were clustered in the amino-terminal domain of the protein. Amino acid sequence of one human liver clone (HL-14) was identical to the rabbit skeletal muscle phosphatase 2A cDNA (with 97% nucleotide identity). The second human liver clone (HL-1) is encoded by a separate gene, and RNA gel blot analysis indicates that both mRNAs are expressed similarly in several human clonal cell lines. Sequence comparison with phosphatase 1 and 2A indicates highly divergent amino acid sequences at the amino and carboxyl termini of the proteins and identifies six highly conserved regions between the two proteins that are predicted to be important for phosphatase enzymatic activity.
- Subjects :
- Macromolecular Substances
Phosphatase
Molecular Sequence Data
Biology
Species Specificity
Complementary DNA
Protein Phosphatase 1
Sequence Homology, Nucleic Acid
Phosphoprotein Phosphatases
Animals
Humans
Amino Acid Sequence
Protein Phosphatase 2
Cloning, Molecular
Gene
Peptide sequence
chemistry.chemical_classification
Multidisciplinary
Base Sequence
Protein primary structure
Nucleotide Mapping
Protein phosphatase 1
Protein phosphatase 2
DNA
DNA Restriction Enzymes
Molecular biology
Amino acid
Biochemistry
chemistry
Genes
Liver
Cats
Cattle
Rabbits
Research Article
Subjects
Details
- ISSN :
- 00278424
- Volume :
- 85
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Accession number :
- edsair.doi.dedup.....7d8ab21f00871d781d9562b59b186c4c