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Effect of bovine serum albumin on the functionality and structure of catanionic surfactant at air–buffer interface
- Source :
- Materials Science and Engineering: C. 33:836-843
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Interaction of bovine serum albumin (BSA) with the solvent spread monolayer of a catanionic surfactant, octadecyltrimethylammonium dodecylsulfate, (C18TA+DS−) at the air–buffer interface was investigated by measuring the surface pressure with time and change in surface area. Dipalmitoylphosphatidylcholine (DPPC) was used as reference. Kinetics of BSA desorption from the interface to the buffer subphase, that of C18TA+DS− and DPPC through their interaction with BSA, were also studied at different BSA concentrations (in the subphase) and surface pressures. Surface pressure (π)–area (A) isotherms (at pH = 5.4, μ = 0.01, T = 298 K) revealed that the coacervate/DPPC monolayer becomes expanded in the presence of BSA at low π while their protein bound species are released into the subphase at high π. Film morphology, studied by epifluorescence microscopy (EFM) and atomic force microscopy (AFM), reveals that the sizes of the domains of both DPPC and coacervate decrease in the presence of BSA. Presence of BSA in the coacervate and DPPC monolayer was supported from AFM data analysis.
- Subjects :
- 1,2-Dipalmitoylphosphatidylcholine
Surface Properties
Analytical chemistry
Bioengineering
Buffers
Microscopy, Atomic Force
Surface pressure
Biomaterials
Surface-Active Agents
chemistry.chemical_compound
Pulmonary surfactant
Cations
Desorption
Monolayer
Animals
Bovine serum albumin
Coacervate
biology
Chemistry
Air
technology, industry, and agriculture
Serum Albumin, Bovine
Hydrogen-Ion Concentration
Solvent
Kinetics
Microscopy, Fluorescence
Mechanics of Materials
Dipalmitoylphosphatidylcholine
biology.protein
Cattle
Subjects
Details
- ISSN :
- 09284931
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Materials Science and Engineering: C
- Accession number :
- edsair.doi.dedup.....7d82c0fb7eb05a6f00242da8d78f66bf