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Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis
- Source :
- Autoantibodies ISBN: 9781493989485
- Publication Year :
- 2019
- Publisher :
- Humana Press, 2019.
-
Abstract
- Deiminated proteins are the target of diagnostic anti-citrullinated peptide/protein autoantibodies (ACPA) in rheumatoid arthritis (RA). Deiminated histone H4 contained in the neutrophil extracellular traps reacts with ACPA, becoming an interesting diagnostic antigen for RA. The identification of the ACPA binding site in histone H4 was performed experimentally by mapping the complete sequence. The method describes the synthesis of an overlapping peptide library covering the entire deiminated sequence of H4 and its further evaluation in ELISA. A detailed description of an ELISA protocol to test RA patients' sera against the synthesized peptides and ACPA is provided.
- Subjects :
- musculoskeletal diseases
0301 basic medicine
Epitope mapping
Peptide
Histone H4
03 medical and health sciences
0302 clinical medicine
Antigen
immune system diseases
Binding site
Rheumatoid arthritis
skin and connective tissue diseases
Peptide library
030203 arthritis & rheumatology
chemistry.chemical_classification
Autoantibody
Neutrophil extracellular traps
Peptide antigen
Epitope mapping, Rheumatoid arthritis, Peptide antigen, ELISA, ACPA, Histone H4
ACPA
Molecular biology
030104 developmental biology
chemistry
ELISA
Subjects
Details
- Language :
- English
- ISBN :
- 978-1-4939-8948-5
- ISBNs :
- 9781493989485
- Database :
- OpenAIRE
- Journal :
- Autoantibodies ISBN: 9781493989485
- Accession number :
- edsair.doi.dedup.....7d82820bdec9074d753ec1e0c924c5fd