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Construction and characterization of an expressed sequenced tag library for the mosquito vector Armigeres subalbatus

Authors :
Nicole T. Perna
Thomas A. Rocheleau
Tze-Tze Liu
Shih-Feng Tsai
George F. Mayhew
Matthew T. Aliota
Hang-Yen Kou
Wen-Long Cho
I-Yu Tsao
Ueng Cheng Yang
Cheng-Chen Chen
Jeremy F. Fuchs
Lyric C. Bartholomay
Chiung-Yen Huang
Bruce M. Christensen
Kwang-Jen Hsiao
Source :
BMC Genomics, Vol 8, Iss 1, p 462 (2007), BMC Genomics
Publication Year :
2007
Publisher :
Springer Science and Business Media LLC, 2007.

Abstract

BackgroundThe mosquito,Armigeres subalbatus, mounts a distinctively robust innate immune response when infected with the nematodeBrugia malayi, a causative agent of lymphatic filariasis. In order to mine the transcriptome for new insight into the cascade of events that takes place in response to infection in this mosquito, 6 cDNA libraries were generated from tissues of adult female mosquitoes subjected to immune-response activation treatments that lead to well-characterized responses, and from aging, naïve mosquitoes. Expressed sequence tags (ESTs) from each library were produced, annotated, and subjected to comparative analyses.ResultsSix libraries were constructed and used to generate 44,940 expressed sequence tags, of which 38,079 passed quality filters to be included in the annotation project and subsequent analyses. All of these sequences were collapsed into clusters resulting in 8,020 unique sequence clusters or singletons. EST clusters were annotated and curated manually within ASAP (A Systematic Annotation Package for Community Analysis of Genomes) web portal according to BLAST results from comparisons to Genbank, and theAnopheles gambiaeandDrosophila melanogastergenome projects.ConclusionThe resulting dataset is the first of its kind for this mosquito vector and provides a basis for future studies of mosquito vectors regarding the cascade of events that occurs in response to infection, and thereby providing insight into vector competence and innate immunity.

Details

ISSN :
14712164
Volume :
8
Database :
OpenAIRE
Journal :
BMC Genomics
Accession number :
edsair.doi.dedup.....7d765ddb85de81e540a6dadfe127c787