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Safety Assessment of Autologous Stem Cell Combination Therapy in Patients With Decompensated Liver Cirrhosis: A Pilot Study
- Source :
- J Clin Exp Hepatol
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Background Haematopoietic stem cell (HSC) infusion has demonstrated short-term improvement in liver functions in patients with chronic liver disease. The combination of HSC with mesenchymal stem cells (MSCs), which has an immunomodulatory effect, may augment the effects and enhance the duration of improvements on liver functions. The aim of the present study was to assess the safety of infusing the combination of autologous HSCs and MSCs in decompensated liver cirrhosis. Methods In phase I of the study, in vitro assessment was performed to observe the effect of coculturing MSCs with HSCs on their viability and cytokine profiles. Phase II of the study was to assess the safety of combination of stem cell infusions. Bone marrow (50 ml) was aspirated for MSC isolation and expansion using standard protocol. Patients received subcutaneous doses (n = 5) of granulocyte colony-stimulating factor (G-CSF) for stem cell mobilization followed by leukapheresis for harvesting HSCs using CliniMacs. HSCs and MSCs were infused through the hepatic artery under fluoroscopic guidance and were monitored for any adverse effects. Results In vitro studies revealed 94% viable HSCs in coculture similar to monoculture. HSCs released only interleukin (IL)-8, whereas MSCs secreted IL-8 and IL-6 in monocultures, and both IL-8 and IL-6 were secreted in coculture. G-CSF administration– and bone marrow aspiration–related complications were not observed. Infusion of the cells through the hepatic artery was safe, and no postprocedural complications were noted. Conclusion The combination of autologous HSC and MSC infusion is a safe procedure in patients with decompensated liver cirrhosis, and the outcomes needed to be assessed in larger studies. Trial number NCT04243681 .
- Subjects :
- Cirrhosis
Hepatology
Combination therapy
business.industry
Mesenchymal stem cell
Leukapheresis
Pharmacology
Chronic liver disease
medicine.disease
03 medical and health sciences
Haematopoiesis
0302 clinical medicine
medicine.anatomical_structure
030220 oncology & carcinogenesis
medicine
Original Article
030211 gastroenterology & hepatology
Bone marrow
Stem cell
business
Subjects
Details
- ISSN :
- 09736883
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical and Experimental Hepatology
- Accession number :
- edsair.doi.dedup.....7d62b40c6dfdd55925ce5c199f691901