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Versicolorin A enhances the genotoxicity of aflatoxin B1 in human liver cells by inducing the transactivation of the Ah-receptor
- Source :
- Budin, C, Man, H Y, Al-Ayoubi, C, Puel, S, van Vugt-Lussenburg, B M A, Brouwer, A, Oswald, I P, van der Burg, B & Soler, L 2021, ' Versicolorin A enhances the genotoxicity of aflatoxin B1 in human liver cells by inducing the transactivation of the Ah-receptor ', Food and Chemical Toxicology, vol. 153, 112258, pp. 1-9 . https://doi.org/10.1016/j.fct.2021.112258, Food and Chemical Toxicology, Food and Chemical Toxicology, Elsevier, 2021, 153, pp.112258. ⟨10.1016/j.fct.2021.112258⟩, Food and Chemical Toxicology, 153:112258, 1-9. Elsevier Limited
- Publication Year :
- 2021
-
Abstract
- International audience; Aflatoxins are a group of mycotoxins that have major adverse effects on human health. Aflatoxin B1 (AFB1) is the most important aflatoxin and a potent carcinogen once converted into a DNA-reactive form by cytochrome P450 enzymes (CYP450). AFB1 biosynthesis involves the formation of Versicolorin A (VerA) which shares structural similarities with AFB1 and can be found in contaminated commodities, often co-occurring with AFB1. This study investigated and compared the toxicity of VerA and AFB1, alone or in combination, in HepG2 human liver cells. Our results show that both toxins have similar cytotoxic effects and are genotoxic although, unlike AFB1, the main genotoxic mechanism of VerA does not involve the formation of DNA double-strand breaks. Additionally, we show that VerA activates the aryl hydrocarbon receptor (AhR) and significantly induce the expression of the CYP450-1A1 (CYP1A1) while AFB1 did not induce AhR-dependent CYP1A1 activation. Combination of VerA with AFB1 resulted in enhanced genotoxic effects, suggesting that AhR-activation by VerA influences AFB1 genotoxicity by promoting its bioactivation by CYP450s to a highly DNA-reactive metabolite. Our results emphasize the need for expanding the toxicological knowledge regarding mycotoxin biosynthetic precursors to identify those who may pose, directly or indirectly, a threat to human health.
- Subjects :
- Transcriptional Activation
Aflatoxin
Aflatoxin B1
[SDV]Life Sciences [q-bio]
Metabolite
Cytotoxicity
Anthraquinones
Toxicology
medicine.disease_cause
03 medical and health sciences
chemistry.chemical_compound
Transactivation
0404 agricultural biotechnology
Cytochrome P-450 Enzyme System
SDG 3 - Good Health and Well-being
hemic and lymphatic diseases
Basic Helix-Loop-Helix Transcription Factors
Versicolorin A
medicine
Humans
Mycotoxin
Carcinogen
030304 developmental biology
0303 health sciences
biology
Chemistry
AhR
Cytochrome P450
Drug Synergism
Hep G2 Cells
04 agricultural and veterinary sciences
General Medicine
Mycotoxins
Aryl hydrocarbon receptor
040401 food science
3. Good health
Receptors, Aryl Hydrocarbon
Biochemistry
biology.protein
Genotoxicity
Mutagens
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 02786915
- Database :
- OpenAIRE
- Journal :
- Budin, C, Man, H Y, Al-Ayoubi, C, Puel, S, van Vugt-Lussenburg, B M A, Brouwer, A, Oswald, I P, van der Burg, B & Soler, L 2021, ' Versicolorin A enhances the genotoxicity of aflatoxin B1 in human liver cells by inducing the transactivation of the Ah-receptor ', Food and Chemical Toxicology, vol. 153, 112258, pp. 1-9 . https://doi.org/10.1016/j.fct.2021.112258, Food and Chemical Toxicology, Food and Chemical Toxicology, Elsevier, 2021, 153, pp.112258. ⟨10.1016/j.fct.2021.112258⟩, Food and Chemical Toxicology, 153:112258, 1-9. Elsevier Limited
- Accession number :
- edsair.doi.dedup.....7d5df3e2d0730fbcd85b809778b47af5