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Flavisolibacter nicotianae sp. nov., isolated from rhizosphere soil of Nicotiana tabacum L
- Source :
- International journal of systematic and evolutionary microbiology. 69(7)
- Publication Year :
- 2019
-
Abstract
- A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain X7XT, was isolated from a rhizosphere soil sample of Nicotiana tabacum L. collected from a tobacco factory located in Kunming, south-western China. The cells showed oxidase-positive and catalase-positive reactions. Growth occurred at 20–40 °C and pH 6.0–8.0, with optimal growth at 30 °C and pH 7.0. The predominant respiratory quinone was MK-7. The major fatty acids were identified as iso-C15 : 0, iso-C17 : 0 3OH and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c). The cellular polar lipids contained phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified glycolipids, four unidentified aminolipids and four unidentified lipids. The genomic DNA G+C content was 49.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain X7XT should be affiliated to the genus Flavisolibacter . Results from further analysis showed that strain X7XT had highest 16S rRNA gene sequence similarity to Flavisolibacter metallilatus TX0661T (96.4 %) and ‘ Flavisolibacter swuensis ’ SR2-4-2T (96.4 %), followed by other species of the genus Flavisolibacter . The polyphasic taxonomic characteristics indicated that strain X7XT represents a novel species of the genus Flavisolibacter , for which the name Flavisolibacter nicotianae sp. nov. (type strain X7XT=KCTC 62326T=CGMCC 16451T) is proposed.
- Subjects :
- 0106 biological sciences
0301 basic medicine
DNA, Bacterial
China
Nicotiana tabacum
010603 evolutionary biology
01 natural sciences
Microbiology
03 medical and health sciences
Genus
RNA, Ribosomal, 16S
Botany
Tobacco
Ecology, Evolution, Behavior and Systematics
Phospholipids
Phylogeny
Soil Microbiology
Rhizosphere
Base Composition
Strain (chemistry)
biology
Phylogenetic tree
Bacteroidetes
Fatty Acids
Vitamin K 2
General Medicine
Sequence Analysis, DNA
biology.organism_classification
16S ribosomal RNA
Bacterial Typing Techniques
genomic DNA
030104 developmental biology
Glycolipids
Bacteria
Subjects
Details
- ISSN :
- 14665034
- Volume :
- 69
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- International journal of systematic and evolutionary microbiology
- Accession number :
- edsair.doi.dedup.....7d57bcdb4b564993687a1c7cdbd41911