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Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins
- Source :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Nature Communications, Vol 11, Iss 1, Pp 1-21 (2020), Nature Communications
- Publication Year :
- 2020
- Publisher :
- Springer Nature, 2020.
-
Abstract
- © The Author(s) 2020.<br />Cell biologists generally consider that microtubules and actin play complementary roles in long- and short-distance transport in animal cells. On the contrary, using melanosomes of melanocytes as a model, we recently discovered that the motor protein myosin-Va works with dynamic actin tracks to drive long-range organelle dispersion in opposition to microtubules. This suggests that in animals, as in yeast and plants, myosin/actin can drive long-range transport. Here, we show that the SPIRE-type actin nucleators (predominantly SPIRE1) are Rab27a effectors that co-operate with formin-1 to generate actin tracks required for myosin-Va-dependent transport in melanocytes. Thus, in addition to melanophilin/myosin-Va, Rab27a can recruit SPIREs to melanosomes, thereby integrating motor and track assembly activity at the organelle membrane. Based on this, we suggest a model in which organelles and force generators (motors and track assemblers) are linked, forming an organelle-based, cell-wide network that allows their collective activity to rapidly disperse the population of organelles long-distance throughout the cytoplasm.<br />This work was supported by a Medical Research Council New Investigator Award to A.N.H. (grant reference G1100063), Wellcome Trust Grant Awards (grant reference 108429/Z/15/Z to E.V.S. and 204843/Z/16/Z to A.N.H.), a Biotechnology and Biological Sciences Research Council (grant reference BB/F016956/1) and University of Nottingham funded PhD studentship awarded to C.L.R. D.A.B. was funded by Biochemical Society Vacation Studentship. L.M. is funded through the Spanish Ministry of Economy. Industry and Competitiveness (MINECO) [BIO2015-70978-R]. E.K. and T.W. are funded by DFG SPP1464, KE 447/10-2” and DFG KE 447/18-1. F.S. is funded by DFG Research Training Group, GRK 2174 Award. N.A. is supported by a PhD studentship from Taibah University, Medina, Kingdom of Saudi Arabia. W.W.K. and E.W.T. are funded by The Royal Society (Newton International Fellowship grant NF161582 to W.W.K.), European Commission (Marie Sklodowska Curie Individual Fellowship grant 752165 to W.W.K.), and Cancer Research UK (C29637/A20183 to E.W.T.). A.K.M. acknowledges financial support of the John and Lucille van Geest Foundation.
- Subjects :
- 0301 basic medicine
82/29
General Physics and Astronomy
Microtubules
14
rab27 GTP-Binding Proteins
13/1
0302 clinical medicine
13/44
Myosin
14/19
lcsh:Science
Cytoskeleton
13/89
Phylogeny
health care economics and organizations
education.field_of_study
Multidisciplinary
Chemistry
article
96/63
3. Good health
Cell biology
Organelle membrane
Melanophilin
actin
Science
Population
education
13/106
13/109
macromolecular substances
General Biochemistry, Genetics and Molecular Biology
Motor protein
03 medical and health sciences
14/33
Humans
Actin
Organelles
Cell Biology
General Chemistry
Actins
HEK293 Cells
030104 developmental biology
Cytoplasm
13/95
lcsh:Q
14/28
631/80/128
631/80
030217 neurology & neurosurgery
631/80/128/1276
Subjects
Details
- ISSN :
- 20411723
- Database :
- OpenAIRE
- Journal :
- Digital.CSIC. Repositorio Institucional del CSIC, instname, Nature Communications, Vol 11, Iss 1, Pp 1-21 (2020), Nature Communications
- Accession number :
- edsair.doi.dedup.....7d5799252f02fde07113db1543c40dc3