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A BIN2-GLK1 Signaling Module Integrates Brassinosteroid and Light Signaling to Repress Chloroplast Development in the Dark
- Source :
- Developmental cell. 56(3)
- Publication Year :
- 2020
-
Abstract
- Arabidopsis GLYCOGEN SYNTHASE KINASE 3 (GSK3)-like kinases play various roles in plant development, including chloroplast development, but the underlying molecular mechanism is not well defined. Here, we demonstrate that transcription factors GLK1 and GLK2 interact with and are phosphorylated by the BRASSINOSTEROID insensitive2 (BIN2). The loss-of-function mutant of BIN2 and its homologs, bin2-3 bil1 bil2, displays abnormal chloroplast development, whereas the gain-of-function mutant, bin2-1, exhibits insensitivity to BR-induced de-greening and reduced numbers of thylakoids per granum, suggesting that BIN2 positively regulates chloroplast development. Furthermore, BIN2 phosphorylates GLK1 at T175, T238, T248, and T256, and mutations of these phosphorylation sites alter GLK1 protein stability and DNA binding and impair plant responses to BRs/darkness. On the other hand, BRs and darkness repress the BIN2-GLK module to enhance BR/dark-mediated de-greening and impair the formation of the photosynthetic apparatus. Our results thus provide a mechanism by which BRs modulate photomorphogenesis and chloroplast development.
- Subjects :
- Chlorophyll
Chloroplasts
Light Signal Transduction
Transcription, Genetic
Mutant
Arabidopsis
Biology
Models, Biological
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Brassinosteroids
Enzyme Stability
Brassinosteroid
Phosphorylation
Molecular Biology
Transcription factor
030304 developmental biology
0303 health sciences
Base Sequence
Arabidopsis Proteins
food and beverages
Cell Biology
Darkness
biology.organism_classification
Cell biology
Chloroplast
Phenotype
chemistry
Thylakoid
Mutation
Photomorphogenesis
Cotyledon
Protein Kinases
030217 neurology & neurosurgery
Developmental Biology
Protein Binding
Transcription Factors
Subjects
Details
- ISSN :
- 18781551
- Volume :
- 56
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Developmental cell
- Accession number :
- edsair.doi.dedup.....7d52c68a7712cb2efa258ed86a9e8c39