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GalNAc-GD1a in human peripheral nerve: Target sites of anti-ganglioside antibody
- Source :
- Neurology. 61:465-470
- Publication Year :
- 2003
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 2003.
-
Abstract
- Background: The authors previously reported that immunoglobulin G (IgG) antibody to the ganglioside N-acetylgalactosaminyl GD1a (GalNAc-GD1a) is associated with the pure motor variant of Guillain-Barre syndrome (GBS). Elucidation of the localization of GalNAc-GD1a in human peripheral nerve tissue may lead to understanding of the pathogenetic role of anti-GalNAc-GD1a antibody in GBS. Methods: IgG anti-GalNAc-GD1a-monospecific antibody was purified from anti-GalNAc-GD1a antibody-positive rabbit sera through an affinity column. Anti-neurofilament-200 monoclonal and anti-HNK-1 monoclonal antibodies were used as the markers for axon and myelin. Immunohistochemical study using double fluorescence labeling technique was conducted in human ventral roots (VR), dorsal roots (DR), intramuscular nerves, and sural nerves. Human teased ventral fibers also were studied. Results: Anti-GalNAc-GD1a antibody immunostained an inner part of compact myelin and additionally a periaxonal-axolemma-related portion in the VR, small-diameter DR fibers, and IM nerves. In sural nerves, small fibers were selectively stained. In VR, the staining was localized in the paranodal region. Conclusion: Anti-GalNAc-GD1a antibodies in patients’ sera may bind to those regions in the VR and IM nerves where GalNAc-GD1a is localized, and may function in the pathogenesis of pure motor type GBS. Further investigation is needed to explain the discrepancy between the immunolocalization of GalNAc-GD1a in sensory nerves and the absence of sensory disturbance in patients with GBS with IgG anti-GalNAc-GD1a antibodies.
- Subjects :
- endocrine system
Pathology
medicine.medical_specialty
medicine.drug_class
Biology
Guillain-Barre Syndrome
Monoclonal antibody
Autoantigens
Immunoglobulin G
Antigen-Antibody Reactions
Epitopes
Myelin
Autoimmune Diseases of the Nervous System
Nerve Fibers
Sural Nerve
Compact myelin
Gangliosides
parasitic diseases
medicine
Animals
Humans
Peripheral Nerves
Axon
Muscle, Skeletal
Autoantibodies
Ganglioside
Antibodies, Monoclonal
carbohydrates (lipids)
medicine.anatomical_structure
Microscopy, Fluorescence
biology.protein
Immunohistochemistry
Female
Immunization
lipids (amino acids, peptides, and proteins)
Rabbits
Neurology (clinical)
Antibody
Spinal Nerve Roots
Subjects
Details
- ISSN :
- 1526632X and 00283878
- Volume :
- 61
- Database :
- OpenAIRE
- Journal :
- Neurology
- Accession number :
- edsair.doi.dedup.....7d3c13cc72e650a5e8f18a1c396237b6