Back to Search
Start Over
Ameliorative Effect of Annona muricata (Graviola) Extract on Hyperglycemia Induced Hepatic Damage in Type 2 Diabetic Mice
- Source :
- Antioxidants, Vol 10, Iss 1546, p 1546 (2021), Antioxidants, Volume 10, Issue 10
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Annona muricata (AM) is evergreen plant of the Annonaceae family and known to have anticancer and antidiabetic effects. However, anti-diabetic mechanisms of AM extracts (AME) associated with hepatic glucose regulation and lipid metabolism remain unclear. In this study, we investigated the protective effect of AME extracted on hepatic damage in diabetic mice. Diabetes was induced by a high-fat diet with two-times streptozotocin (STZ) injection (60 mg/kg BW) in C57BL/6 male mice. The diabetic mice were daily administered with AME (50 or 100 mg/kg BW) by gavage for 9 weeks. Biomarkers related to energy metabolism and insulin signaling were examined to identify the effect of AME on hyperglycemia induced hepatic damage. AME supplementation reduced levels of FBG, HbA1c, HOMA-IR and hepatic lipid profiles as well as enhanced insulin signaling by increased the protein levels of IRS-1 accompanied GLUT2 in diabetic mice. Especially low dose of AME showed the beneficial effect of reducing oxidative stress (4-HNE, protein carbonyls, Nrf2, NQO1) and improved hepatic morphology demonstrated by lipid droplets along with upregulation of lipophagy (pAMPK, p-mTOR/mTOR, LC3-2/LC3-1) in diabetic mice. Moreover, AME supplementation ameliorated hepatic lipid metabolism (FAS, SREBP1c, C/EBPα, PPARγ, CPT1A, PPARα) and energy metabolism (pAMPK, PGC1α) in diabetic mice. Taken together, this study suggested that AME could be helpful to prevent hepatic abnormality by regulation of insulin signaling associated with energy metabolism and autophagy in diabetes.
- Subjects :
- medicine.medical_specialty
autophagy
graviola
Physiology
Clinical Biochemistry
RM1-950
medicine.disease_cause
Biochemistry
Diabetes mellitus
Lipid droplet
Internal medicine
medicine
AMPK/Akt-mTOR signaling
lipophagy
Molecular Biology
Annona muricata
biology
Chemistry
type 2 diabetes mellitus (T2DM)
Lipid metabolism
Cell Biology
medicine.disease
biology.organism_classification
Streptozotocin
Insulin receptor
Endocrinology
biology.protein
GLUT2
Therapeutics. Pharmacology
hepatic lipid steatosis
Oxidative stress
non-alcoholic fatty liver disease (NAFLD)
medicine.drug
Subjects
Details
- Language :
- English
- ISSN :
- 20763921
- Volume :
- 10
- Issue :
- 1546
- Database :
- OpenAIRE
- Journal :
- Antioxidants
- Accession number :
- edsair.doi.dedup.....7d36130ba5da59cf4d0a645a9e7a5bee