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Altered Umbilical Cord Blood Nutrient Levels, Placental Cell Turnover and Transporter Expression in Human Term Pregnancies Conceived by Intracytoplasmic Sperm Injection (ICSI)
- Source :
- Nutrients, Volume 13, Issue 8, Nutrients, Vol 13, Iss 2587, p 2587 (2021)
- Publication Year :
- 2021
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2021.
-
Abstract
- Assisted reproductive technologies (ART) may increase risk for abnormal placental development, preterm delivery and low birthweight. We investigated placental morphology, transporter expression and paired maternal/umbilical fasting blood nutrient levels in human term pregnancies conceived naturally (n = 10) or by intracytoplasmic sperm injection (ICSI<br />n = 11). Maternal and umbilical vein blood from singleton term (&gt<br />37 weeks) C-section pregnancies were assessed for levels of free amino acids, glucose, free fatty acids (FFA), cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), very low-density lipoprotein (VLDL) and triglycerides. We quantified placental expression of GLUT1 (glucose), SNAT2 (amino acids), P-glycoprotein (P-gp) and breast cancer resistance protein (BCRP) (drug) transporters, and placental morphology and pathology. Following ICSI, placental SNAT2 protein expression was downregulated and umbilical cord blood levels of citrulline were increased, while FFA levels were decreased at term (p &lt<br />0.05). Placental proliferation and apoptotic rates were increased in ICSI placentae (p &lt<br />0.05). No changes in maternal blood nutrient levels, placental GLUT1, P-gp and BCRP expression, or placental histopathology were observed. In term pregnancies, ICSI impairs placental SNAT2 transporter expression and cell turnover, and alters umbilical vein levels of specific nutrients without changing placental morphology. These may represent mechanisms through which ICSI impacts pregnancy outcomes and programs disease risk trajectories in offspring across the life course.
- Subjects :
- 0301 basic medicine
Very low-density lipoprotein
Amino Acid Transport System A
free fatty acids (FFA)
Reproductive technology
Umbilical cord
Umbilical vein
chemistry.chemical_compound
0302 clinical medicine
High-density lipoprotein
Pregnancy
ATP Binding Cassette Transporter, Subfamily G, Member 2
TX341-641
glucose
reproductive and urinary physiology
Glucose Transporter Type 1
030219 obstetrics & reproductive medicine
Nutrition and Dietetics
biology
Pregnancy Outcome
apoptosis
Lipid
Fetal Blood
Amino acid
Neoplasm Proteins
medicine.anatomical_structure
Low-density lipoprotein
Premature Birth
Female
intracytoplasmic sperm injection (ICSI)
Human
Adult
assisted reproductive technology (ART)
Reproductive Techniques, Assisted
placenta
Pregnancy Trimester, Third
proliferation
Article
Andrology
Neoplasm Protein
lipids
03 medical and health sciences
Placenta
medicine
Humans
Sperm Injections, Intracytoplasmic
Cell Proliferation
SNAT2
amino acids
business.industry
Nutrition. Foods and food supply
Apoptosi
Nutrients
030104 developmental biology
chemistry
Fertilization
citrulline
biology.protein
GLUT1
P-gp and BCRP
business
Food Science
Nutrient
Subjects
Details
- Language :
- English
- ISSN :
- 20726643
- Database :
- OpenAIRE
- Journal :
- Nutrients
- Accession number :
- edsair.doi.dedup.....7d1b00c5e78beb3f8210296118eb6576
- Full Text :
- https://doi.org/10.3390/nu13082587