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Structural and biophysical characterization of the α-carbonic anhydrase from the gammaproteobacterium Thiomicrospira crunogena XCL-2: insights into engineering thermostable enzymes for CO2 sequestration
- Source :
- Acta crystallographica. Section D, Biological crystallography. 71(Pt 8)
- Publication Year :
- 2015
-
Abstract
- Biocatalytic CO2sequestration to reduce greenhouse-gas emissions from industrial processes is an active area of research. Carbonic anhydrases (CAs) are attractive enzymes for this process. However, the most active CAs display limited thermal and pH stability, making them less than ideal. As a result, there is an ongoing effort to engineer and/or find a thermostable CA to fulfill these needs. Here, the kinetic and thermal characterization is presented of an α-CA recently discovered in the mesophilic hydrothermal vent-isolate extremophileThiomicrospira crunogenaXCL-2 (TcruCA), which has a significantly higher thermostability compared with human CA II (melting temperature of 71.9°Cversus59.5°C, respectively) but with a tenfold decrease in the catalytic efficiency. The X-ray crystallographic structure of the dimeric TcruCA shows that it has a highly conserved yet compact structure compared with other α-CAs. In addition, TcruCA contains an intramolecular disulfide bond that stabilizes the enzyme. These features are thought to contribute significantly to the thermostability and pH stability of the enzyme and may be exploited to engineer α-CAs for applications in industrial CO2sequestration.
- Subjects :
- Models, Molecular
Protein Conformation
Crystallography, X-Ray
Protein Engineering
Protein structure
Structural Biology
Carbonic anhydrase
Catalytic Domain
Enzyme Stability
Extremophile
Humans
Thermostability
Carbonic Anhydrases
chemistry.chemical_classification
biology
Chemistry
Temperature
General Medicine
Protein engineering
Carbon Dioxide
biology.organism_classification
Lyase
Combinatorial chemistry
Research Papers
Enzyme
Biochemistry
biology.protein
Biocatalysis
Protein Multimerization
Gammaproteobacteria
Thiomicrospira crunogena
Subjects
Details
- ISSN :
- 13990047
- Volume :
- 71
- Issue :
- Pt 8
- Database :
- OpenAIRE
- Journal :
- Acta crystallographica. Section D, Biological crystallography
- Accession number :
- edsair.doi.dedup.....7d11b9e83bede1abfe6bca0e35c8af95