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Human meibum chain branching variability with age, gender and meibomian gland dysfunction
- Source :
- The Ocular Surface. 17:327-335
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- NMR spectroscopy was used to measure hydrocarbon chain branching variability in meibum with age, gender and Meibomian gland dysfuction (MGD). A cohort of 65 meibum donors without dry eye and 31 donors with MGD was studied. Heteronuclear 2D NMR was used to confirm CH3 resonance assignments allowing us to positively identify 23 of the 27 proton resonances of cholesteryl ester and 1H resonances due to straight chain, iso-branched and anteiso-branched hydrocarbons. Meibum from donors without dry eye contained 57 ± 1% straight-chains, 23 ± 1% iso-branched chains and 20 ± 1% anteiso-branched hydrocarbon chains. Compared with meibum from donors without dry eye, meibum from donors with MGD contained less, 50 ± 2% straight-chains, more, 32 ± 2% iso-branched chains and the same amount, 18.0 ± 0.07%, of anteiso-branched hydrocarbon chains. Meibum hydrocarbon chain branching did not change with age between 22 and 68 years of age, nor was it influenced by gender. Based on previous studies, one would expect anteiso-branched chains would contribute to lowering the phase transition temperature of meibum, decrease the elasticity of the tear film lipid layer and increase the molecular area and spreading of meibum on the surface of the eye. Although we speculate that the observed differences in iso-chain branching do not influence tear film stability or rheology, the speculation has yet to be tested.
- Subjects :
- Adult
Male
0301 basic medicine
Aging
Magnetic Resonance Spectroscopy
Phase transition temperature
Adolescent
Meibomian gland
Branching (polymer chemistry)
Article
Young Adult
03 medical and health sciences
0302 clinical medicine
Nuclear magnetic resonance
Straight chain
medicine
Humans
Child
Meibomian Gland Dysfunction
Aged
Aged, 80 and over
Chemistry
Meibomian gland dysfunction
Infant
Meibomian Glands
Middle Aged
Lipid Metabolism
Lipids
Ophthalmology
030104 developmental biology
medicine.anatomical_structure
Child, Preschool
Tears
030221 ophthalmology & optometry
Female
sense organs
Biomarkers
Subjects
Details
- ISSN :
- 15420124
- Volume :
- 17
- Database :
- OpenAIRE
- Journal :
- The Ocular Surface
- Accession number :
- edsair.doi.dedup.....7d08214f38f2a764069043dbaf684808