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Epitope specificity and relative clonal abundance do not affect CD8 differentiation patterns during lymphocytic choriomeningitis virus infection
- Source :
- Journal of Virology, Journal of Virology, American Society for Microbiology, 2009, 83 (22), pp.11795-807. ⟨10.1128/JVI.01402-09⟩, Journal of Virology, American Society for Microbiology, 2009, 83 (22), pp.11795-807. 〈10.1128/JVI.01402-09〉, Europe PubMed Central, Journal of Virology, 2009, 83 (22), pp.11795-807. ⟨10.1128/JVI.01402-09⟩
- Publication Year :
- 2009
- Publisher :
- HAL CCSD, 2009.
-
Abstract
- To evaluate the impact of immunodominance on CD8 T-cell properties, we compared the functional properties of dominant and subdominant populations in the response to lymphocytic choriomeningitis virus (LCMV). To improve functional discrimination, in addition to the usual tests of phenotype and function, we used a sensitive technique that allows the screening of all CD8 effector genes simultaneously in single cells. Surprisingly, these methods failed to reveal a major impact of clonal dominance in CD8 properties throughout the response. Aiming to increase clonal dominance, we examined high-frequency transferred P14 T-cell receptor transgenic (TCR Tg) cells. Under these conditions LCMV is cleared faster, and accordingly we found an accelerated response. However, when Tg and endogenous cells were studied in the same mice, where they should be subjected to the same antigen load, they showed overlapping properties, and the presence of P14 cells did not modify endogenous responses to other LCMV epitopes or a perturbed immunodominance hierarchy in the memory phase. Using allotype-labeled Tg cells, we found that during acute infection up to 80% downregulated their TCR and were undetectable by tetramer binding, and that tetramer-negative and tetramer-positive cells had very different features. Since Tg cells are not available to evaluate immune responses in humans and, in many cases, are not available from the mouse, the tetramer-based evaluation of early immune responses in most situations of high viremia may be incomplete and biased.
- Subjects :
- MESH: Lymphocytic Choriomeningitis
Immunology
Receptors, Antigen, T-Cell
Epitopes, T-Lymphocyte
Immunodominance
CD8-Positive T-Lymphocytes
Lymphocytic Choriomeningitis
Biology
Lymphocytic choriomeningitis
Microbiology
Epitope
MESH: Epitopes, T-Lymphocyte
Mice
03 medical and health sciences
0302 clinical medicine
Immune system
Antigen
Virology
Virus latency
medicine
CD8 T cells, clonal abundance, T cell differentiation, lymphocytic choriomeningitis virus infection
Animals
Lymphocytic choriomeningitis virus
MESH: Animals
[SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
MESH: Mice
030304 developmental biology
0303 health sciences
T-cell receptor
MESH: Virus Latency
MESH: Receptors, Antigen, T-Cell
medicine.disease
MESH: CD8-Positive T-Lymphocytes
MESH: Gene Expression Regulation
Virus Latency
Gene Expression Regulation
Insect Science
MESH: Immunologic Memory
Pathogenesis and Immunity
MESH: Lymphocytic choriomeningitis virus
Immunologic Memory
CD8
[SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
030215 immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022538X and 10985514
- Database :
- OpenAIRE
- Journal :
- Journal of Virology, Journal of Virology, American Society for Microbiology, 2009, 83 (22), pp.11795-807. ⟨10.1128/JVI.01402-09⟩, Journal of Virology, American Society for Microbiology, 2009, 83 (22), pp.11795-807. 〈10.1128/JVI.01402-09〉, Europe PubMed Central, Journal of Virology, 2009, 83 (22), pp.11795-807. ⟨10.1128/JVI.01402-09⟩
- Accession number :
- edsair.doi.dedup.....7cff61fb19542cac6196ec36419d2f57