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Siderocalin inhibits the intracellular replication ofMycobacterium tuberculosisin macrophages
- Source :
- FEMS Immunology & Medical Microbiology. 58:138-145
- Publication Year :
- 2010
- Publisher :
- Oxford University Press (OUP), 2010.
-
Abstract
- Siderocalin is a secreted protein that binds to siderophores to prevent bacterial iron acquisition. While it has been shown to inhibit the growth of Mycobacterium tuberculosis (M.tb) in extracellular cultures, its effect on this pathogen within macrophages is not clear. Here, we show that siderocalin expression is up-regulated following M.tb infection of mouse macrophage cell lines and primary murine alveolar macrophages. Furthermore, siderocalin added exogenously as a recombinant protein or over-expressed in the RAW264.7 macrophage cell line inhibited the intracellular growth of the pathogen. A variant form of siderocalin, which is expressed only in the macrophage cytosol, inhibited intracellular M.tb growth as effectively as the normal, secreted form, an observation that provides mechanistic insight into how siderocalin might influence iron acquisition by the bacteria in the phagosome. Our findings are consistent with an important role for siderocalin in protection against M.tb infection and suggest that exogenously administered siderocalin may have therapeutic applications in tuberculosis.
- Subjects :
- Microbiology (medical)
Siderophore
Immunology
Siderocalin
Biology
Microbiology
Article
Cell Line
Mice
Lipocalin-2
Macrophages, Alveolar
Extracellular
Animals
Humans
Immunology and Allergy
Macrophage
Lung
Tuberculosis, Pulmonary
Cell Proliferation
Phagosome
Cell growth
Mycobacterium tuberculosis
General Medicine
Lipocalins
Mice, Inbred C57BL
Infectious Diseases
Cell culture
Mutation
Female
Carrier Proteins
Intracellular
Subjects
Details
- ISSN :
- 1574695X and 09288244
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- FEMS Immunology & Medical Microbiology
- Accession number :
- edsair.doi.dedup.....7cf68a261b53106a15afcdaded6c5052