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Label-Free Immunodetection in High Ionic Strength Solutions Using Carbon Nanotube Transistors with Nanobody Receptors
- Source :
- Proceedings, Vol 1, Iss 4, p 491 (2017)
- Publication Year :
- 2017
- Publisher :
- MDPI, 2017.
-
Abstract
- Nanomaterial-based field-effect transistors (FETs) have been proposed for real-time, label-free detection of various biological species. However, screening of the analyte charge by electrolyte ions (Debye screening) has so far limited their use in physiological samples. Here, this challenge is overcome by combining FETs based on single-walled semiconducting carbon nanotube networks (SWCNTs) with a novel surface functionalization comprising: (1) short nanobody receptors, and (2) a polyethylene glycol layer (PEG). Nanobodies are stable, easy-to-produce, short biological receptors (~2–4 nm) that enable analyte binding closer to the sensor surface. The addition of PEG enhances the signal in high ionic strength environment. Using green fluorescent protein (GFP) as a model antigen, high selectivity and sub-picomolar detection limit with a dynamic range exceeding 4 orders of magnitude is demonstrated in physiological solutions. The presented immunoassay is fast, label-free, does not require any sample pre-treatment or washing steps.
- Subjects :
- Detection limit
Analyte
Materials science
polyethylene glycol (PEG)
single-walled carbon nanotube (SWCNT) networks
lcsh:A
Nanotechnology
Polyethylene glycol
Carbon nanotube
Electrolyte
Debye screening
field-effect transistor (FET)
Carbon nanotube field-effect transistor
law.invention
label-free immunosensing
chemistry.chemical_compound
nanobodies (VHH)
chemistry
Chemical engineering
Ionic strength
law
Surface modification
lcsh:General Works
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- Proceedings of Eurosensors 2017, Paris, France, 3–6 September 2017
- Accession number :
- edsair.doi.dedup.....7ce59b7a2db4cbb7beab41fa90b25a38