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Metagenomic insights into microbial metabolism affecting arsenic dispersion in Mediterranean marine sediments
- Source :
- Molecular Ecology, Molecular Ecology, Wiley, 2013, 22 (19), pp.4870-4883. ⟨10.1111/mec.12432⟩, Molecular Ecology, 2013, 22 (19), pp.4870-4883. ⟨10.1111/mec.12432⟩
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Chantier qualité GA; International audience; Microorganisms dwelling in sediments have a crucial role in biogeochemical cycles and are expected to have a strong influence on the cycle of arsenic, a metalloid responsible for severe water pollution and presenting major health risks for human populations. We present here a metagenomic study of the sediment from two harbours on the Mediterranean French coast, l'Estaque and St Mandrier. The first site is highly polluted with arsenic and heavy metals, while the arsenic concentration in the second site is below toxicity levels. The goal of this study was to elucidate the potential impact of the microbial community on the chemical parameters observed in complementary geochemical studies performed on the same sites. The metagenomic sequences, along with those from four publicly available metagenomes used as control data sets, were analysed with the RAMMCAP workflow. The resulting functional profiles were compared to determine the over-represented Gene Ontology categories in the metagenomes of interest. Categories related to arsenic resistance and dissimilatory sulphate reduction were over-represented in l'Estaque. More importantly, despite very similar profiles, the identification of specific sequence markers for sulphate-reducing bacteria and sulphur-oxidizing bacteria showed that sulphate reduction was significantly more associated with l'Estaque than with St Mandrier. We propose that biotic sulphate reduction, arsenate reduction and fermentation may together explain the higher mobility of arsenic observed in l'Estaque in previous physico-chemical studies of this site. This study also demonstrates that it is possible to draw sound conclusions from comparing complex and similar unassembled metagenomes at the functional level, even with very low sequence coverage
- Subjects :
- Geologic Sediments
Biogeochemical cycle
Microorganism
Microbial metabolism
arsenic pollution
chemistry.chemical_element
Biology
Arsenic
03 medical and health sciences
chemistry.chemical_compound
Proteobacteria
Mediterranean Sea
Genetics
Water Pollutants
14. Life underwater
metagenomic
Water pollution
Ecology, Evolution, Behavior and Systematics
030304 developmental biology
[SDV.EE]Life Sciences [q-bio]/Ecology, environment
0303 health sciences
Sulfur-Reducing Bacteria
Sulfates
030306 microbiology
Ecology
Arsenate
marine sediment
Sequence Analysis, DNA
6. Clean water
microbial metabolism
Gene Ontology
chemistry
Microbial population biology
Genes, Bacterial
13. Climate action
Metagenomics
Metagenome
France
Subjects
Details
- Language :
- English
- ISSN :
- 09621083 and 1365294X
- Database :
- OpenAIRE
- Journal :
- Molecular Ecology, Molecular Ecology, Wiley, 2013, 22 (19), pp.4870-4883. ⟨10.1111/mec.12432⟩, Molecular Ecology, 2013, 22 (19), pp.4870-4883. ⟨10.1111/mec.12432⟩
- Accession number :
- edsair.doi.dedup.....7cdc33cce392e16549793d47bdee99b9