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Catabolism of cysteine, cystine, cysteinesulfinate, and OTC by isolated perfused rat hindquarter
- Source :
- The American journal of physiology. 264(5 Pt 1)
- Publication Year :
- 1993
-
Abstract
- The metabolism of cysteine and related compounds was investigated in the isolated perfused hindquarter of the rat. An erythrocyte-based perfusion medium was used; use of a perfluorochemical emulsion, FC-43, resulted in apparent chemical oxidation of cysteine, whereas bovine erythrocytes did not appear to contribute significantly to the metabolism of cysteine. Rat skeletal muscle perfused with L-[35S]cysteine, L-[35S]cystine, L-[35S]cysteinesulfinate, or L-2-oxo-[35S]thiazolidine-4-carboxylate (OTC) for 2 h produced [35S]sulfate and [35S]taurine. In all cases, the partitioning of cysteine or cysteinesulfinate between metabolism to taurine and sulfate was similar, suggesting that cysteine metabolism in hindquarter may occur via formation and catabolism of cysteinesulfinate by either cysteinesulfinate decarboxylase or aspartate (cysteine-sulfinate) aminotransferase. However, the activity of cysteine dioxygenase was extremely low, suggesting that the conversion of cysteine to cysteinesulfinate may have been non-enzymatic.
- Subjects :
- Male
medicine.medical_specialty
Taurine
Radioisotope Dilution Technique
Pyrrolidonecarboxylic Acid
Phosphocreatine
Physiology
Endocrinology, Diabetes and Metabolism
Cystine
In Vitro Techniques
Sulfur Radioisotopes
Rats, Sprague-Dawley
chemistry.chemical_compound
Physiology (medical)
Internal medicine
medicine
Animals
Cysteine
Pyruvates
Cysteine metabolism
Neurotransmitter Agents
biology
Catabolism
Chemistry
Adenine Nucleotides
Sulfates
Muscles
Cysteine dioxygenase
Metabolism
Hindlimb
Rats
Perfusion
Thiazoles
Endocrinology
Biochemistry
biology.protein
Lactates
Thiazolidines
Subjects
Details
- ISSN :
- 00029513
- Volume :
- 264
- Issue :
- 5 Pt 1
- Database :
- OpenAIRE
- Journal :
- The American journal of physiology
- Accession number :
- edsair.doi.dedup.....7cabd301115a36161cd3544ef89e1156