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A new type 1 ribosome-inactivating protein from the seeds of Gypsophila elegans M.Bieb
- Source :
- Phytochemistry. 157
- Publication Year :
- 2018
-
Abstract
- Ribosome-inactivating proteins (RIPs) are enzymes with N-glycosylase activity that remove adenine bases from the ribosomal RNA. In theory, one single RIP molecule internalized into a cell is sufficient to induce cell death. For this reason, RIPs are of high potential as toxic payload for anti-tumor therapy. A considerable number of RIPs are synthesized by plants that belong to the carnation family (Caryophyllaceae). Prominent examples are the RIPs saporin from Saponaria officinalis L. or dianthin from Dianthus caryophyllus L. In this study, we have isolated and characterized a novel RIP (termed gypsophilin-S) from the tiny seeds of Gypsophila elegans M. Bieb. (Caryophyllaceae). It is noteworthy that this is the first study presenting the complete amino acid sequence of a RIP from a Gypsophila species. Gypsophilin-S was isolated from the defatted seed material following ammonium sulphate precipitation and HPLC-based ion exchange chromatography. Gypsophilin-S-containing fractions were analysed by SDS-PAGE and mass spectrometry. The full amino acid sequence of gypsophilin-S was assembled by MALDI-TOF-MS-MS and PCR. Gypsophilin-S exhibited strong adenine releasing activity and its cytotoxicity in human glioblastoma cells was investigated using an impedance-based real-time assay in comparison with recombinant saporin and dianthin.
- Subjects :
- 0106 biological sciences
Models, Molecular
endocrine system
Gypsophila
Saporin
Protein Conformation
Caryophyllaceae
Plant Science
Horticulture
01 natural sciences
Biochemistry
Cell Line, Tumor
Humans
Saponaria officinalis
Amino Acid Sequence
Molecular Biology
Peptide sequence
Gypsophila elegans
biology
010405 organic chemistry
Chemistry
Ribosome-inactivating protein
General Medicine
Ribosomal RNA
biology.organism_classification
Antineoplastic Agents, Phytogenic
Saporins
0104 chemical sciences
Seeds
biology.protein
010606 plant biology & botany
Subjects
Details
- ISSN :
- 18733700
- Volume :
- 157
- Database :
- OpenAIRE
- Journal :
- Phytochemistry
- Accession number :
- edsair.doi.dedup.....7c9dd373efaee7252df53803ef6b6946