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Penicillin susceptibility among invasive MSSA infections: a multicentre study in 16 Spanish hospitals
- Source :
- Zaguán. Repositorio Digital de la Universidad de Zaragoza, Universitat Politècnica de Catalunya (UPC)
- Publication Year :
- 2021
- Publisher :
- Oxford University Press (OUP), 2021.
-
Abstract
- Objectives To determine the prevalence of penicillin susceptibility among MSSA causing bloodstream infections (BSIs) in 16 Spanish hospitals and to characterize the penicillin-susceptible MSSA (MSSA-PENS) isolates. Methods A total of 1011 Staphylococcus aureus isolates were collected from blood cultures in 16 Spanish hospitals during 2018–19 (6–12 months) and their susceptibility to 18 antimicrobials was determined. The MSSA-PENS isolates were selected and examined by PCR to determine the presence of the blaZ gene, other resistance genes and the genes lukF/lukS-PV, eta, etb and tst. The immune evasion cluster (IEC) type was also analysed. All the MSSA-PENS isolates were submitted to S. aureus protein A (spa) typing and the clonal complexes (CCs) were assigned according to their spa type. Results The prevalence of MSSA was 74.6% (754/1011) and 14.9% (151/1011) were MSSA-PENS-blaZnegative. MSSA-PENS-blaZnegative isolates (n = 151) were ascribed to 88 spa types and 11 CCs. The most frequent CCs were CC5 (35/151) and CC398 (25/151), with t002-CC5 and t571-CC398 being the most common lineages. Pan-susceptibility was identified in 117 of the 151 MSSA-PENS-blaZnegative isolates (77.5%). In the remaining isolates, erythromycin and clindamycin resistance was the most frequent resistance found, although tobramycin, ciprofloxacin, fusidic acid, mupirocin and/or tetracycline resistance was also detected. Thirty-eight MSSA-PENS-blaZnegative isolates were IEC negative and four isolates were Panton–Valentine leucocidin (‘PVL’) positive. Conclusions A high penicillin susceptibility rate was detected among MSSA, opening therapeutic opportunities for BSIs. The emergence of new successful MSSA-PENS clones could be responsible for these data. The detection among MSSA-PENS-blaZnegative isolates of the clonal lineage CC398 or the absence of an IEC raises questions about their possible animal origin, requiring further analysis.
- Subjects :
- Methicillin-Resistant Staphylococcus aureus
0301 basic medicine
Microbiology (medical)
Staphylococcus aureus
Fusidic acid
030106 microbiology
Erythromycin
Mupirocin
Microbial Sensitivity Tests
Penicillins
Biology
medicine.disease_cause
Microbiology
03 medical and health sciences
chemistry.chemical_compound
medicine
Animals
Pharmacology (medical)
Typing
Pharmacology
Tetracycline Resistance
Staphylococcal Infections
biochemical phenomena, metabolism, and nutrition
bacterial infections and mycoses
Hospitals
Anti-Bacterial Agents
Ciprofloxacin
Penicillin
030104 developmental biology
Infectious Diseases
chemistry
Methicillin Susceptible Staphylococcus Aureus
medicine.drug
Subjects
Details
- ISSN :
- 14602091 and 03057453
- Volume :
- 76
- Database :
- OpenAIRE
- Journal :
- Journal of Antimicrobial Chemotherapy
- Accession number :
- edsair.doi.dedup.....7c901ed434cc3f1b73b9e3c0ff3c07e3