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Development of a LC-MS/MS method for determination of propofol-glucuronide in hair and preliminary study on relationships between dose and hair concentration
- Source :
- Forensic science international. 306
- Publication Year :
- 2019
-
Abstract
- Propofol abuse has been reported worldwide, suggesting the need to establish analytical methods for human biological samples to investigate the abuse of propofol. This study aimed to investigate the relationship between dose and hair concentration using a simple and rapid analytical method developed and validated in this study. In the sample preparation, hair samples were washed with distilled water and methanol and extracted in methanol during 16h at room temperature. After centrifugation and evaporation, the residue was reconstituted and filtered through a 0.22μm membrane filter before LC-MS/MS analysis. The precursor-to-product ion transitions were 353 → 175, 113 for propofol glucuronide and m/z 370 → 175, 113 for internal standard(propofol glucuronide-d17). The calibration curves were satisfactory (R2=0.9997) and the limits of detection and quantification were 2 and 5pg/mg, respectively. In addition, this study collected the history of propofol use from subjects using a questionnaire and analyzed subjects' hair samples using a validated analytical method. As a result, the concentrations of propofol glucuronide ranged from 7 to 122pg/mg (mean : 51pg/mg). There were cases of positive relationships, but generally there was no correlation between dose and hair concentration.
- Subjects :
- Adult
Male
Calibration curve
Substance-Related Disorders
01 natural sciences
Pathology and Forensic Medicine
03 medical and health sciences
Forensic Toxicology
Young Adult
0302 clinical medicine
Glucuronides
Tandem Mass Spectrometry
medicine
Humans
Hypnotics and Sedatives
Sample preparation
Centrifugation
030216 legal & forensic medicine
Propofol
Detection limit
Residue (complex analysis)
Chromatography
Chemistry
010401 analytical chemistry
Hair analysis
Middle Aged
0104 chemical sciences
Substance Abuse Detection
Distilled water
Female
Law
medicine.drug
Chromatography, Liquid
Hair
Subjects
Details
- ISSN :
- 18726283
- Volume :
- 306
- Database :
- OpenAIRE
- Journal :
- Forensic science international
- Accession number :
- edsair.doi.dedup.....7c8c3cf6ccf39e41f98ff7f71c4d3fa4