Back to Search
Start Over
Ubiquitin Proteasome-dependent Degradation of the Transcriptional Coactivator PGC-1α via the N-terminal Pathway
- Source :
- Journal of Biological Chemistry. 285:40192-40200
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- PGC-1α is a potent, inducible transcriptional coactivator that exerts control on mitochondrial biogenesis and multiple cellular energy metabolic pathways. PGC-1α levels are controlled in a highly dynamic manner reflecting regulation at both transcriptional and post-transcriptional levels. Here, we demonstrate that PGC-1α is rapidly degraded in the nucleus (t(½ 0.3 h) via the ubiquitin proteasome system. An N-terminal deletion mutant of 182 residues, PGC182, as well as a lysine-less mutant form, are nuclear and rapidly degraded (t(½) 0.5 h), consistent with degradation via the N terminus-dependent ubiquitin subpathway. Both PGC-1α and PGC182 degradation rates are increased in cells under low serum conditions. However, a naturally occurring N-terminal splice variant of 270 residues, NT-PGC-1α is cytoplasmic and stable (t(½7 h), providing additional evidence that PGC-1α is degraded in the nucleus. These results strongly suggest that the nuclear N terminus-dependent ubiquitin proteasome pathway governs PGC-1α cellular degradation. In contrast, the cellular localization of NT-PCG-1α results in a longer-half-life and possible distinct temporal and potentially biological actions.
- Subjects :
- Cytoplasm
Proteasome Endopeptidase Complex
Biology
Protein degradation
Biochemistry
Mice
Ubiquitin
Transcriptional regulation
Animals
Humans
Amino Acid Sequence
Molecular Biology
Transcription factor
Heat-Shock Proteins
Cellular localization
Sequence Deletion
Cell Nucleus
Protein Stability
Hep G2 Cells
Cell Biology
Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
Protein Structure, Tertiary
Ubiquitin ligase
Mitochondrial biogenesis
Proteasome
Protein Synthesis and Degradation
Trans-Activators
biology.protein
HeLa Cells
Transcription Factors
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 285
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....7c8a2ed7196f127d6fe70545290c3abe