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Centrifugation-induced production of triacylglycerols in Chlamydomonas reinhardtii
- Source :
- Bioresource Technology Reports, Bioresource Technology Reports, 2019, 5, pp.326-330. ⟨10.1016/j.biteb.2018.10.003⟩, Bioresource Technology Reports, Elsevier, 2019, 5, pp.326-330. ⟨10.1016/j.biteb.2018.10.003⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- We found that Chlamydomonas reinhardtii, a model microalga, produces triacylglycerols (TAGs) when the cell pellet is left overnight after centrifugation. The centrifuged cells produce three times more TAGs than those cultured in a nitrogen (N) depletion condition in the first 24 h. The chloroplast membranes of the centrifuged cells are largely disrupted. In addition, the accumulated TAGs contain only trivial amount of C18:1 (11) and C18:3 (5,9,12) fatty acids that are more abundant in TAGs accumulated under N-starved condition. Overall, TAGs that are induced by centrifugation contain more saturated fatty acids than that induced by N starvation. These suggest that the centrifugation triggers the production of TAGs through yet unknown mechanism. An importance of this finding is that not only quantity but also quality of the TAG production can be modified by alternating environmental stimuli rather than genetic means.
- Subjects :
- Environmental Engineering
biology
Renewable Energy, Sustainability and the Environment
Chemistry
020209 energy
[SDV]Life Sciences [q-bio]
Chlamydomonas
food and beverages
Chlamydomonas reinhardtii
Bioengineering
02 engineering and technology
010501 environmental sciences
biology.organism_classification
01 natural sciences
Chloroplast
Cell Pellet
Membrane
Biochemistry
0202 electrical engineering, electronic engineering, information engineering
Centrifugation
Waste Management and Disposal
ComputingMilieux_MISCELLANEOUS
0105 earth and related environmental sciences
Subjects
Details
- Language :
- English
- ISSN :
- 2589014X
- Database :
- OpenAIRE
- Journal :
- Bioresource Technology Reports, Bioresource Technology Reports, 2019, 5, pp.326-330. ⟨10.1016/j.biteb.2018.10.003⟩, Bioresource Technology Reports, Elsevier, 2019, 5, pp.326-330. ⟨10.1016/j.biteb.2018.10.003⟩
- Accession number :
- edsair.doi.dedup.....7c5e86751f098bd3d9aa7b3be994878f