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Generation of Cost-Effective Paper-Based Tissue Models through Matrix-Assisted Sacrificial 3D Printing
- Source :
- Nano Lett
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- Due to the combined advantages of cellulose and nanoscale (diameter 20–60 nm), bacterial cellulose possesses a series of attractive features including its natural origin, moderate biosynthesis process, good biocompatibility, and cost-effectiveness. Moreover, bacterial cellulose nanofibers can be conveniently processed into three-dimensional (3D) intertwined structures and form stable paper devices after simple drying. These advantages make it suitable as the material for construction of organ-on-a-chip devices using matrix-assisted sacrificial 3D printing. We successfully fabricated various microchannel structures embedded in the bulk bacterial cellulose hydrogels and retained their integrity after the drying process. Interestingly, these paper-based devices containing hollow microchannels could be rehydrated and populated with relevant cells to form vascularized tissue models. As a proof-of-concept demonstration, we seeded human umbilical vein endothelial cells (HUVECs) into the microchannels to obtain the vasculature and inoculated the MCF-7 cells onto the surrounding matrix of the paper device to build a 3D paper-based vascularized breast tumor model. The results showed that the microchannels were perfusable, and both HUVECs and MCF-7 cells exhibited favorable proliferation behaviors. This study may provide a new strategy for constructing simple and low-cost in vitro tissue models, which may find potential applications in drug screening and personalized medicine.
- Subjects :
- Paper
Materials science
Biocompatibility
Cell Survival
Nanofibers
3D printing
Bioengineering
Nanotechnology
02 engineering and technology
Matrix (biology)
Article
chemistry.chemical_compound
Human Umbilical Vein Endothelial Cells
Humans
General Materials Science
Cellulose
Microchannel
Tissue Engineering
Tissue Scaffolds
business.industry
Mechanical Engineering
Polysaccharides, Bacterial
Bioprinting
Equipment Design
General Chemistry
021001 nanoscience & nanotechnology
Condensed Matter Physics
chemistry
Bacterial cellulose
Nanofiber
Printing, Three-Dimensional
Self-healing hydrogels
MCF-7 Cells
0210 nano-technology
business
Subjects
Details
- ISSN :
- 15306992 and 15306984
- Volume :
- 19
- Database :
- OpenAIRE
- Journal :
- Nano Letters
- Accession number :
- edsair.doi.dedup.....7c4b062188d74b7d87680f1b5b444428