The design and operation of a dual-beam long-focal-length fluorometer for monitoring the oxidative metabolismin vivo

In 1962, Chanceet al. demonstrated the feasibility of monitoring intramitochondrial NADH levelsin vivo. To extend this capability to the neurosurgical operating room on a practical basis, and to enable a comparison of metabolic changes in two different cortical areas a dual-beam long-focal-length fluorometer for the direct measurement of NADH fluorescence changes was designed.