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Hepatitis B virus DNA integration in tumour tissue of a non-cirrhotic HFE-haemochromatosis patient with hepatocellular carcinoma
- Publication Year :
- 2013
-
Abstract
- Co-existence of multiple causes of liver injury increases the risk of hepatocellular carcinoma (HCC) development. HCC usually develops in patients with cirrhosis although it may also occur in individuals with no or mild liver disease, in particular in cases with hepatitis B virus (HBV) infection.Here we report the case of a 43year-old man with HFE-haemochromatosis, seronegative for hepatitis B and C infections, who developed HCC in the absence of severe liver damage. Both tumoural and non-tumoural liver DNA extracts were tested by nested-PCR and primers specific for four different HBV genomic regions in order to evaluate the presence of occult HBV infection. Only X gene sequences were detected in tumour (but not in non-tumour) DNA extracts. HBV-Alu PCR showed a HBV integration involving a 5′-deleted X gene with an intact enhancer-II/basal-core promoter region. The viral-host junction sequencing revealed that this integrant was located upstream of the partitioning-defective-6-homolog-gamma gene (PARD6G) and real time-PCR quantification demonstrated that PARD6G was overexpressed in tumour compared to non-tumour liver tissues.In conclusion, the combination of HFE-haemochromatosis and occult HBV infection in this patient might have led to a sequel of cellular events that determined the development of HCC even in the absence of cirrhosis.
- Subjects :
- Adult
Liver Cirrhosis
Male
HBsAg
Pathology
medicine.medical_specialty
Carcinoma, Hepatocellular
Cirrhosis
Hepatocellular carcinoma
Virus Integration
Hepatitis C virus
Hepatocellular carcinoma, HFE-haemochromatosis, HBV X-gene, HBV DNA integration, Partitioning-defective-6-homolog-gamma gene
HBV DNA integration
medicine.disease_cause
HFE-haemochromatosis
Liver disease
Hepatocellular carcinoma,HFE-haemochromatosis,HBV X-gene,HBV DNA integration,Partitioning-defective-6-homolog-gamma gene
Humans
Medicine
Hemochromatosis
Adaptor Proteins, Signal Transducing
Hepatitis B virus
Hepatology
business.industry
Liver Neoplasms
Hepatitis B
medicine.disease
digestive system diseases
DNA, Viral
Partitioning-defective-6-homolog-gamma gene
business
HBV X-gene
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....7c3255523e926ed5a4ab6bfcd2adc5d0