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NDAT suppresses pro-inflammatory gene expression to enhance resveratrol-induced anti-proliferation in oral cancer cells
- Source :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 136
- Publication Year :
- 2019
-
Abstract
- Nano-diamino-tetrac (NDAT), a tetraiodothyroxine deaminated nano-particulated analog, has shown to inhibit expression of pro-inflammatory genes. NDAT inhibits expression of programmed death-ligand 1 (PD-L1). On the other hand, in addition to inhibiting inflammatory effect, the stilbene, resveratrol induces expression of cyclooxygenase-2 (COX-2) and its accumulation. Sequentially, inducible COX-2 complexes with p53 and induces p53-dependent anti-proliferation. In current study, we investigated mechanisms involved in combined treatment of NDAT and resveratrol on anti-proliferation in human oral cancer cells. Both resveratrol and NDAT inhibited expression of pro-inflammatory IL-1β and TNF-α. They also inhibited expression of CCND1 and PD-L1. Both resveratrol and NDAT induced BAD expression but only resveratrol induced COX-2 expression in both OEC-M1 and SCC-25 cells. Combined treatment attenuated gene expression significantly compared with resveratrol treatment in both cancer cell lines. Resveratrol reduced nuclear PD-L1 accumulation which was enhanced by a STAT3 inhibitor, S31-201 or NDAT suggesting that NDAT may inactivate STAT3 to inhibit PD-L1 accumulation. In the presence of T4, NDAT further enhanced resveratrol-induced anti-proliferation in both cancer cell lines. These findings provide a novel understanding of the inhibition of NDAT in thyroxine-induced pro-inflammatory effect on resveratrol-induced anticancer properties.
- Subjects :
- STAT3 Transcription Factor
Gene Expression
Resveratrol
Toxicology
B7-H1 Antigen
03 medical and health sciences
chemistry.chemical_compound
0404 agricultural biotechnology
Cyclin D1
Cell Line, Tumor
Gene expression
Humans
STAT3
Gene
Polyglactin 910
Inflammatory genes
030304 developmental biology
Cell Proliferation
0303 health sciences
biology
Drug Synergism
04 agricultural and veterinary sciences
General Medicine
Anti proliferative
040401 food science
Thyroxine
chemistry
Cyclooxygenase 2
Cancer cell
Cancer research
biology.protein
Mouth Neoplasms
Food Science
Subjects
Details
- ISSN :
- 18736351
- Volume :
- 136
- Database :
- OpenAIRE
- Journal :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Accession number :
- edsair.doi.dedup.....7c2979fcdc4ec48667d6c0fc578a7538