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Identification and Characterization of Various Cholecystokinin B Receptor mRNA Forms in Rat Brain Tissue and Partial Determination of the Cholecystokinin B Receptor Gene Structure
- Source :
- Journal of Neurochemistry, Journal of Neurochemistry, Wiley, 1994, 63 (4), pp.1199-1206. ⟨10.1046/j.1471-4159.1994.63041199.x⟩, Journal of Neurochemistry, 1994, 63 (4), pp.1199-1206. ⟨10.1046/j.1471-4159.1994.63041199.x⟩
- Publication Year :
- 1994
- Publisher :
- HAL CCSD, 1994.
-
Abstract
- Using the powerful method of DNA amplification, we have been able to isolate several cholecystokinin B (CCKB) receptor (CCKB-R) mRNA forms from rat brain tissue, allowing the detection of a truncated mRNA species and the determination of the CCKB-R gene structure. Unspliced precursor mRNA and the mature form were identified in the cerebral cortex, hypothalamus, and hippocampus in apparently differing proportions according to the region examined, suggesting that the expression of the CCKB-R could be modulated at a posttranscriptional level. In the case of the cerebellum, only a completely unspliced mRNA form was detected, in agreement with previous studies in which CCKB ligand binding sites were not observed. In contrast, a truncated CCKB-R mRNA, lacking 250 bp, was detected in all the studied brain regions except for the cerebellum. This mRNA, for which a cellular function has not been assigned, potentially encodes a protein consisting of 168 amino acids.
- Subjects :
- Molecular Sequence Data
Hypothalamus
Gene Expression
In situ hybridization
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Biochemistry
Hippocampus
Polymerase Chain Reaction
03 medical and health sciences
Cellular and Molecular Neuroscience
0302 clinical medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
RNA, Messenger
Receptor
Gene
In Situ Hybridization
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
Cholecystokinin
DNA Primers
Cerebral Cortex
0303 health sciences
Messenger RNA
Base Sequence
Brain
Molecular biology
Receptor, Cholecystokinin B
Rats
Organ Specificity
RNA splicing
Cholecystokinin B receptor
Receptors, Cholecystokinin
Precursor mRNA
hormones, hormone substitutes, and hormone antagonists
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 00223042 and 14714159
- Database :
- OpenAIRE
- Journal :
- Journal of Neurochemistry, Journal of Neurochemistry, Wiley, 1994, 63 (4), pp.1199-1206. ⟨10.1046/j.1471-4159.1994.63041199.x⟩, Journal of Neurochemistry, 1994, 63 (4), pp.1199-1206. ⟨10.1046/j.1471-4159.1994.63041199.x⟩
- Accession number :
- edsair.doi.dedup.....7c2001f719af069ac52db3b296e0ea75