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Topical Spilanthol Inhibits MAPK Signaling and Ameliorates Allergic Inflammation in DNCB-Induced Atopic Dermatitis in Mice
- Source :
- International Journal of Molecular Sciences, Vol 20, Iss 10, p 2490 (2019), International Journal of Molecular Sciences, Volume 20, Issue 10
- Publication Year :
- 2019
- Publisher :
- MDPI AG, 2019.
-
Abstract
- Atopic dermatitis (AD) is a recurrent allergic skin disease caused by genetic and environmental factors. Patients with AD may experience immune imbalance, increased levels of mast cells, immunoglobulin (Ig) E and pro-inflammatory factors (Cyclooxygenase, COX-2 and inducible NO synthase, iNOS). While spilanthol (SP) has anti-inflammatory and analgesic activities, its effect on AD remains to be explored. To develop a new means of SP, inflammation-related symptoms of AD were alleviated, and 2,4-dinitrochlorobenzene (DNCB) was used to induce AD-like skin lesions in BALB/c mice. Histopathological analysis was used to examine mast cells and eosinophils infiltration in AD-like skin lesions. The levels of IgE, IgG1 and IgG2a were measured by enzyme-linked immunosorbent assay (ELISA) kits. Western blot was used for analysis of the mitogen-activated protein kinase (MAPK) pathways and COX-2 and iNOS protein expression. Topical SP treatment reduced serum IgE and IgG2a levels and suppressed COX-2 and iNOS expression via blocked mitogen-activated protein kinase (MAPK) pathways in DNCB-induced AD-like lesions. Histopathological examination revealed that SP reduced epidermal thickness and collagen accumulation and inhibited mast cells and eosinophils infiltration into the AD-like lesions skin. These results indicate that SP may protect against AD skin lesions through inhibited MAPK signaling pathways and may diminish the infiltration of inflammatory cells to block allergic inflammation.
- Subjects :
- MAPK/ERK pathway
MAP Kinase Signaling System
Polyunsaturated Alkamides
Anti-Inflammatory Agents
Immunoglobulins
Nitric Oxide Synthase Type II
Immunoglobulin E
Article
Catalysis
Dermatitis, Atopic
Allergic inflammation
Inorganic Chemistry
lcsh:Chemistry
Mice
Western blot
Anti-Allergic Agents
Dinitrochlorobenzene
allergic inflammation
Animals
Medicine
Physical and Theoretical Chemistry
Protein kinase A
Molecular Biology
lcsh:QH301-705.5
Spectroscopy
Mice, Inbred BALB C
biology
medicine.diagnostic_test
atopic dermatitis
business.industry
Organic Chemistry
General Medicine
Atopic dermatitis
medicine.disease
MAPK
Computer Science Applications
lcsh:Biology (General)
lcsh:QD1-999
Cyclooxygenase 2
Immunology
biology.protein
spilanthol
Female
IgE
Antibody
business
Infiltration (medical)
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 20
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....7c186aaec467f5929c3aa3726c7df564