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Biological properties of solid free form designed ceramic scaffolds with BMP-2: in vitro and in vivo evaluation
- Source :
- PLoS ONE, Vol 7, Iss 3, p e34117 (2012), PLoS ONE, Repisalud, Instituto de Salud Carlos III (ISCIII)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- Porous ceramic scaffolds are widely studied in the tissue engineering field due to their potential in medical applications as bone substitutes or as bone-filling materials. Solid free form (SFF) fabrication methods allow fabrication of ceramic scaffolds with fully controlled pore architecture, which opens new perspectives in bone tissue regeneration materials. However, little experimentation has been performed about real biological properties and possible applications of SFF designed 3D ceramic scaffolds. Thus, here the biological properties of a specific SFF scaffold are evaluated first, both in vitro and in vivo, and later scaffolds are also implanted in pig maxillary defect, which is a model for a possible application in maxillofacial surgery. In vitro results show good biocompatibility of the scaffolds, promoting cell ingrowth. In vivo results indicate that material on its own conducts surrounding tissue and allow cell ingrowth, thanks to the designed pore size. Additional osteoinductive properties were obtained with BMP-2, which was loaded on scaffolds, and optimal bone formation was observed in pig implantation model. Collectively, data show that SFF scaffolds have real application possibilities for bone tissue engineering purposes, with the main advantage of being fully customizable 3D structures. This work was supported by Ministerio de Ciencia e Innovación (PET2008-0168, CSD2009-00088), and Ministerio de Educación y Ciencia y Fondo Social Europeo (PTQ05-02-02498 and Fundación PCM, MAT 2006-08720). These funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Escherichia-coli-produced recombinant human BMP-2 (rhBMP-2) was generously supplied by Noricum S.L. (Spain). We thank Alba Cuenca-Moya (Noricum S.L.) and Virginia Gutierrez (Pharmakine) for their technical support. Sí
- Subjects :
- Scaffold
Ceramics
Bone Regeneration
Anatomy and Physiology
Swine
Bone Morphogenetic Protein 2
lcsh:Medicine
Biocompatible Materials
Bone tissue
Biochemistry
Mice
Engineering
Tissue engineering
Maxilla
Materials Design
Ceramic
lcsh:Science
Musculoskeletal System
Multidisciplinary
Bone Transplantation
Anatomy
medicine.anatomical_structure
visual_art
Models, Animal
visual_art.visual_art_medium
Medicine
Rabbits
Porosity
Research Article
Biotechnology
Materials science
Biocompatibility
Materials Science
Bioengineering
Bone morphogenetic protein 2
Bone and Bones
Cell Line
Biomaterials
Rheumatology
medicine
Cell Adhesion
Animals
Bone regeneration
Muscle, Skeletal
Biology
Tissue Engineering
Regeneration (biology)
lcsh:R
Alkaline Phosphatase
Metabolism
lcsh:Q
Tomography, X-Ray Computed
Biomedical engineering
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....7c13b24c8612a1cacf705d954ecf5b45