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Droplet Digital PCR-Based Detection and Quantification of GyrA Thr-86-Ile Mutation Based Fluoroquinolone-Resistant Campylobacter jejuni
- Source :
- Microbiology Spectrum. 10
- Publication Year :
- 2022
- Publisher :
- American Society for Microbiology, 2022.
-
Abstract
- Fluoroquinolone (FQ)-resistant Campylobacter jejuni is a serious problem worldwide that limits effective treatment of infections. The traditional detection method depends on bacterial isolation and MIC testing, or traditional PCR, which is time-consuming and hard to identify the FQ-resistant C. jejuni in a high abundance wild-type background. This study aimed to develop a rapid and accurate ddPCR assay to detect FQ-resistant C. jejuni mutants based on the crucial resistance mutation C257T (Thr-86-Ile) in
- Subjects :
- Microbiology (medical)
General Immunology and Microbiology
Ecology
Physiology
Campylobacter
Microbial Sensitivity Tests
Cell Biology
Real-Time Polymerase Chain Reaction
Anti-Bacterial Agents
Gastroenteritis
Campylobacter jejuni
Infectious Diseases
DNA Gyrase
Drug Resistance, Bacterial
Mutation
Genetics
Animals
Chickens
Fluoroquinolones
Subjects
Details
- ISSN :
- 21650497
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Microbiology Spectrum
- Accession number :
- edsair.doi.dedup.....7bc7692feb4d006a85053cd20716d9af