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Starch-derived absorbable polysaccharide hemostat enhances bone healing via BMP-2 protein
- Source :
- Acta histochemica. 119(3)
- Publication Year :
- 2016
-
Abstract
- Surgical hemostasis is critical in reducing the likelihood of excessive bleeding and blood transfusion. In treating some cases, commonly used hemostatic agent showed limited efficacy and prolonged degradation and clearance, causing an inhibition of bone healing. Starch absorbable polysaccharide (SAPH) is a novel hemostatic agent made from a plant starch, which can be completely absorbed and achieve better hemostatic effects than many commonly used hemostatic agents. However, whether SAPH can induce a promotion of bone healing remains unknown. In this study, we used a model of rabbit parietal bone defect and a mouse osteoblast cell line MC3T3-E1 to evaluate the effects of SAPH on bone healing. We found that SAPH significantly decreased bone healing scores, reduced defective area of parietal bone, and increased the areas of bone trabeculae and cavitas medullaris. In addition, SAPH enhanced MC3T3-E1 osteoblasts proliferation, up-regulated the expressions of alkaline phosphatase (ALP) and osteocalcin and increased the level of bone morphogenetic protein 2 (BMP-2) in MC3T3-E1 osteoblasts. These SAPH-induced benefits in MC3T3-E1 osteoblasts were significantly abolished by the application of BMP-2-siRNA. These findings suggested that SAPH enhances bone healing, promotes the proliferation, differentiation and maturation of osteoblast by up-regulating BMP-2 expression in osteoblastic cells.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Histology
Osteocalcin
Bone Morphogenetic Protein 2
02 engineering and technology
Bone healing
Bone morphogenetic protein
Bone morphogenetic protein 2
Cell Line
Parietal Bone
03 medical and health sciences
Mice
Internal medicine
Absorbable Implants
medicine
Animals
Cell Proliferation
Hemostatic Agent
Osteoblasts
biology
Chemistry
Osteoblast
Cell Differentiation
Starch
Cell Biology
General Medicine
021001 nanoscience & nanotechnology
Alkaline Phosphatase
Hemostasis, Surgical
Surgery
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Gene Expression Regulation
biology.protein
Bone Trabeculae
Rabbits
0210 nano-technology
Parietal bone
Subjects
Details
- ISSN :
- 16180372
- Volume :
- 119
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Acta histochemica
- Accession number :
- edsair.doi.dedup.....7bc5617a40e88477a1d226964118fc1d