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Pretreatment of spent mushroom substrate for enhancing the conversion of fermentable sugar
- Source :
- Bioresource Technology. 148:596-600
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- To develop a cost-effective biopesticide, spent mushroom substrate (SMS) extract was studied as a potential carbon source for cultivating Bacillus thuringiensis (Bt). Several pretreatments were compared to determine the optimal method for degrading cellulose to produce reducing sugars, including dilute sulfuric acid (0.5-2.0% v/v, 50-121°C, 1h), sodium hydroxide (0.5-2% w/v, 50-121°C, 1h), calcium hydroxide (0.2-4% w/v, 50-121°C, 1h), and hot water (50-121°C, 1h). Pretreatment was followed by standard enzymatic hydrolysis and fermentation. Results showed that the highest cellulose degradation was obtained using 2% dilute sulfuric acid pretreatment at 121°C for 1h, resulting in a high yield of reducing sugar (284.24 g/kg SMS). Sporulation was also highest using the same pretreatment. Use of SMS is not only an alternative way to commercialize Bt-based biopesticide, but also a potential solution for the environmental pollution associated with accumulation of the spent substrate of the mushroom industry.
- Subjects :
- Hot Temperature
Environmental Engineering
Bacillus thuringiensis
Carbohydrates
Bioengineering
Environmental pollution
Calcium Hydroxide
Hydrolysis
chemistry.chemical_compound
Enzymatic hydrolysis
Sodium Hydroxide
Food science
Cellulose
Waste Management and Disposal
Spores, Bacterial
Waste Products
chemistry.chemical_classification
Renewable Energy, Sustainability and the Environment
Chemistry
Monosaccharides
Water
Agriculture
Sulfuric acid
General Medicine
Sulfuric Acids
Reducing sugar
Biochemistry
Sodium hydroxide
Fermentation
Agaricales
Biotechnology
Subjects
Details
- ISSN :
- 09608524
- Volume :
- 148
- Database :
- OpenAIRE
- Journal :
- Bioresource Technology
- Accession number :
- edsair.doi.dedup.....7bc06395aeb9794d571509c44ca91e89