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Upregulation of phosphoserine phosphatase contributes to tumor progression and predicts poor prognosis in nonā€small cell lung cancer patients

Authors :
Huajian Yu
Xiaoyu Ji
Mengxi Ge
Li Liao
Xiaohua Liang
Qiong Zhan
Ruofan Huang
Xinli Zhou
Source :
Thoracic Cancer
Publication Year :
2019
Publisher :
Wiley, 2019.

Abstract

Background Growing evidence indicates that high phosphoserine phosphatase (PSPH) expression is associated with tumor prognosis in many types of cancers. However, the role of PSPH in non-small cell lung cancer (NSCLC) is unclear. The purpose of this study was to investigate the clinical significance of PSPH in NSCLC. Methods One hundred forty-three patients with histologically confirmed NSCLC who underwent surgery were included. Quantitative real-time PCR and Western blot were used to assess PSPH expression in paired tumor and corresponding adjacent non-tumorous tissues. The role of PSPH in invasion and cell growth was investigated in vitro. Results Compared to adjacent normal lung tissues, PSPH messenger RNA and protein levels were significantly higher in NSCLC tissues, and the PSPH expression level was positively related to clinical stage, metastasis, and recurrence. High PSPH expression was predictive of poor overall survival. A549 cells transfected with small interfering-PSPH showed inhibited cell migration, invasion, and proliferation. We further demonstrated that PSPH might promote the invasive capabilities of NSCLC cells through the AKT/AMPK signaling pathway. Conclusion Our results indicate that PSPH may act as a putative oncogene in NSCLC, and may be a vital molecular marker for the metastasis and proliferation of NSCLC cells by regulating the AKT/AMPK signaling pathway.

Details

ISSN :
17597714 and 17597706
Volume :
10
Database :
OpenAIRE
Journal :
Thoracic Cancer
Accession number :
edsair.doi.dedup.....7badb6d564ab6ef92ab39a7a7e567ef8