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Upregulation of phosphoserine phosphatase contributes to tumor progression and predicts poor prognosis in nonāsmall cell lung cancer patients
- Source :
- Thoracic Cancer
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Background Growing evidence indicates that high phosphoserine phosphatase (PSPH) expression is associated with tumor prognosis in many types of cancers. However, the role of PSPH in non-small cell lung cancer (NSCLC) is unclear. The purpose of this study was to investigate the clinical significance of PSPH in NSCLC. Methods One hundred forty-three patients with histologically confirmed NSCLC who underwent surgery were included. Quantitative real-time PCR and Western blot were used to assess PSPH expression in paired tumor and corresponding adjacent non-tumorous tissues. The role of PSPH in invasion and cell growth was investigated in vitro. Results Compared to adjacent normal lung tissues, PSPH messenger RNA and protein levels were significantly higher in NSCLC tissues, and the PSPH expression level was positively related to clinical stage, metastasis, and recurrence. High PSPH expression was predictive of poor overall survival. A549 cells transfected with small interfering-PSPH showed inhibited cell migration, invasion, and proliferation. We further demonstrated that PSPH might promote the invasive capabilities of NSCLC cells through the AKT/AMPK signaling pathway. Conclusion Our results indicate that PSPH may act as a putative oncogene in NSCLC, and may be a vital molecular marker for the metastasis and proliferation of NSCLC cells by regulating the AKT/AMPK signaling pathway.
- Subjects :
- Male
0301 basic medicine
Lung Neoplasms
Kaplan-Meier Estimate
AMP-Activated Protein Kinases
NSCLC
Metastasis
0302 clinical medicine
Cell Movement
Carcinoma, Non-Small-Cell Lung
Cell Cycle
Cell migration
General Medicine
Middle Aged
Prognosis
Up-Regulation
Gene Expression Regulation, Neoplastic
PSPH
Oncology
030220 oncology & carcinogenesis
Original Article
Female
Signal Transduction
Adult
Pulmonary and Respiratory Medicine
proliferation
AKT/AMPK signaling pathway
03 medical and health sciences
Cell Line, Tumor
Biomarkers, Tumor
medicine
metastasis
Humans
Lung cancer
Protein kinase B
Aged
Cell Proliferation
Neoplasm Staging
A549 cell
Oncogene
business.industry
Cell growth
Original Articles
medicine.disease
Phosphoric Monoester Hydrolases
respiratory tract diseases
030104 developmental biology
Tumor progression
Cancer research
business
Proto-Oncogene Proteins c-akt
Subjects
Details
- ISSN :
- 17597714 and 17597706
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Thoracic Cancer
- Accession number :
- edsair.doi.dedup.....7badb6d564ab6ef92ab39a7a7e567ef8