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Invariant Chain–independent Function of H-2M in the Formation of Endogenous Peptide–Major Histocompatibility Complex Class II Complexes In Vivo
- Source :
- The Journal of Experimental Medicine
- Publication Year :
- 1998
- Publisher :
- The Rockefeller University Press, 1998.
-
Abstract
- Efficient loading of major histocompatibility complex class II molecules with peptides requires the invariant chain (Ii) and the class II–like molecule H-2M. Recent in vitro biochemical studies suggest that H2-M may function as a chaperone to rescue empty class II dimers. To test this hypothesis in vivo, we generated mice lacking both Ii and H-2M (Ii−/−M−/−). Antigen presenting cells (APCs) from Ii−/−M−/− mice, as compared with APCs from Ii−/− mice, exhibit a significant reduction in their ability to present self-peptides to a panel of class II I-Ab–restricted T cells. As a consequence of this defect in the loading of self peptides, CD4+ thymocyte development is profoundly impaired in Ii−/−M−/− mice, resulting in a peripheral CD4+ T cell population with low levels of T cell receptor expression. These findings are consistent with the idea that H-2M functions as a chaperone in the peptide loading of class II molecules in vivo.
- Subjects :
- CD4-Positive T-Lymphocytes
Macromolecular Substances
T cell
Immunology
Population
Antigen presentation
Biology
Autoantigens
Article
03 medical and health sciences
Mice
0302 clinical medicine
In vivo
medicine
Immunology and Allergy
Animals
education
Antigen-presenting cell
030304 developmental biology
Mice, Knockout
0303 health sciences
education.field_of_study
Antigen Presentation
T-cell receptor
H-2 Antigens
Histocompatibility Antigens Class II
Cell Differentiation
Articles
Molecular biology
In vitro
Cell biology
Antigens, Differentiation, B-Lymphocyte
Mice, Inbred C57BL
Thymocyte
medicine.anatomical_structure
Peptides
030215 immunology
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 15409538 and 00221007
- Volume :
- 187
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....7ba7ccf9603fcb1adb42527245b0c384