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Phosphorylation of basic fibroblast growth factor (FGF-2) in the nuclei of SK-Hep-1 cells
- Source :
- FEBS Letters. 331:228-232
- Publication Year :
- 1993
- Publisher :
- Wiley, 1993.
-
Abstract
- The subcellular fractions containing protein kinases capable of phosphorylating basic fibroblast growth factor (FGF-2) are unknown, but having previously characterized one that is associated with the plasma membrane [1991, Mol. Endocrinol. 5, 1003310121 we evaluated the catalytic properties of another in the nucleus. The reaction is time (linear up to 15 min), enzyme (2,000-25,000 nuclei/ml), and substrate (X, 0.18 ,uM) dependent, and the targets serine. DNase pretreatment of nuclei decreases the incorporation of phosphate into FGF-2 by 50% and the reaction. It is also inhibited by heparin (EC, 1 pug/ml) and spermidine (EC,, 3 PM). Calcium and CAMP have no effect. We conclude that the kinase is distinct from PKA, and PKC, and suggest that changes in glycosaminoglycan and polyamine concentrations during the cell cycle may modulate FGF-2 phosphorylation in the nucleus, or as it is translocated to the nucleus. Growth factor; Nucleus; Kinase; Phosphorylation; FGF; SK-Hep cell
- Subjects :
- Kinase
Spermidine
medicine.medical_treatment
Basic fibroblast growth factor
Biophysics
In Vitro Techniques
Biology
Fibroblast growth factor
Biochemistry
Nucleus
Mice
chemistry.chemical_compound
Structural Biology
Cyclic AMP
Tumor Cells, Cultured
Genetics
medicine
FGF
Animals
Humans
Phosphorylation
Protein kinase A
Molecular Biology
Protein kinase C
Cell Nucleus
Heparin
Growth factor
3T3 Cells
Cell Biology
Molecular biology
medicine.anatomical_structure
chemistry
SK-Hep cell
Fibroblast Growth Factor 2
Protein Kinases
Subcellular Fractions
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 331
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....7b93b5df4c949e040cf5bbda0d059dfa