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A digitalized isothermal nucleic acid testing platform based on a pump-free open droplet array microfluidic chip
- Source :
- The Analyst. 146:6960-6969
- Publication Year :
- 2021
- Publisher :
- Royal Society of Chemistry (RSC), 2021.
-
Abstract
- Digital PCR has shown great potential for quantitative nucleic acid testing (NAT), but most existing platforms are dependent on large auxiliary equipment (e.g., vacuum pump, amplification instrument, fluorescence microscope) to achieve target dispersion, amplification, signal capture and result analysis. Such complex, expensive and bulky NAT platforms have limited their applications in resource-limited areas, especially for point-of-care testing (POCT). In this work, we designed a digital isothermal NAT platform based on a pump-free open droplet array microfluidic chip. A pump-free microfluidic chip was developed based on an open microdroplet array in the form of thousands of independent microdroplets for spontaneous sample dispersion, without the need for external power. Combined with a handheld fluorescent signal reader based on a smartphone, this digital NAT platform can accurately quantify as low as 1 copy per μL of λDNA. Therefore, our integrated NAT platform, as a potable, robust and low-cost tool for highly accurate NA quantitative analysis, holds great potential for POCT applications.
- Subjects :
- Computer science
business.industry
Microfluidics
Microfluidic Analytical Techniques
Nucleic Acid Testing
Biochemistry
Signal
Isothermal process
Analytical Chemistry
law.invention
Microfluidic chip
law
Nat
Lab-On-A-Chip Devices
Nucleic Acids
Electrochemistry
Environmental Chemistry
Vacuum pump
Digital polymerase chain reaction
business
Nucleic Acid Amplification Techniques
Spectroscopy
Computer hardware
Subjects
Details
- ISSN :
- 13645528 and 00032654
- Volume :
- 146
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....7b779e71ed8c847ed4f6f7c9d864c153