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Comprehensive identification of lysine 2âhydroxyisobutyrylated proteins in Ustilaginoidea virens reveals the involvement of lysine 2âhydroxyisobutyrylation in fungal virulence
- Source :
- Journal of Integrative Plant Biology. 63:409-425
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Lysine 2-hydroxyisobutyrylation (Khib ) is a newly identified post-translational modification (PTM) that plays important roles in transcription and cell proliferation in eukaryotes. However, its function remains unknown in phytopathogenic fungi. Here, we performed a comprehensive assessment of Khib in the rice false smut fungus Ustilaginoidea virens, using Tandem Mass Tag (TMT)-based quantitative proteomics approach. A total of 3 426 Khib sites were identified in 977 proteins, suggesting that Khib is a common and complex PTM in U. virens. Our data demonstrated that the 2-hydroxyisobutyrylated proteins are involved in diverse biological processes. Network analysis of the modified proteins revealed a highly interconnected protein network that included many well-studied virulence factors. We confirmed that the Zn-binding reduced potassium dependency3-type histone deacetylase (UvRpd3) is a major enzyme that removes 2-hydroxyisobutyrylation and acetylation in U. virens. Notably, mutations of Khib sites in the mitogen-activated protein kinase (MAPK) UvSlt2 significantly reduced fungal virulence and decreased the enzymatic activity of UvSlt2. Molecular dynamics simulations demonstrated that 2-hydroxyisobutyrylation in UvSlt2 increased the hydrophobic solvent-accessible surface area and thereby affected binding between the UvSlt2 enzyme and its substrates. Our findings thus establish Khib as a major post-translational modification in U. virens and point to an important role for Khib in the virulence of this phytopathogenic fungus.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Lysine
Quantitative proteomics
Hydroxybutyrates
Virulence
Plant Science
Molecular Dynamics Simulation
01 natural sciences
Biochemistry
General Biochemistry, Genetics and Molecular Biology
Fungal Proteins
03 medical and health sciences
Amino Acid Sequence
Protein Interaction Maps
Protein kinase A
chemistry.chemical_classification
biology
Ustilaginoidea virens
biology.organism_classification
030104 developmental biology
Enzyme
chemistry
Acetylation
Hypocreales
Mutation
Histone deacetylase
010606 plant biology & botany
Subjects
Details
- ISSN :
- 17447909 and 16729072
- Volume :
- 63
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Plant Biology
- Accession number :
- edsair.doi.dedup.....7b65db29d8994b7106a4845575b223bb