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Hypersensitive response to Potato virus Y in potato cultivar Sárpo Mira is conferred by the Ny-Smira gene located on the long arm of chromosome IX
- Source :
- Molecular Breeding
- Publication Year :
- 2014
- Publisher :
- Springer Netherlands, 2014.
-
Abstract
- Potato virus Y (PVY, Potyvirus) is the fifth most important plant virus worldwide in terms of economic and scientific impact. It infects members of the family Solanaceae and causes losses in potato, tomato, tobacco, pepper and petunia production. In potato and its wild relatives, two types of resistance genes against PVY have been identified. While Ry genes confer symptomless extreme resistance, Ny genes cause a hypersensitive response visible as local necrosis that may also be able to prevent the virus from spreading under certain environmental conditions. The potato cultivar Sarpo Mira originates from Hungary and is highly resistant to PVY, although the source of this resistance remains unknown. We show that cv. Sarpo Mira reacts with a hypersensitive response leading to necrosis after PVYNTN infection in detached leaf, whole plant and grafting assays. The hypersensitivity to PVYNTN segregated amongst 140 individuals of tetraploid progeny of cvs. Sarpo Mira × Maris Piper in a 1:1 ratio, indicating that it was conferred by a single, dominant gene in simplex. Moreover, we identified five DNA markers linked to this trait and located the underlying locus (Ny-Smira) to the long arm of potato chromosome IX. This position corresponds to the location of the Ry chc and Ny-1 genes for PVY resistance. A simple PCR marker, located 1 cM from the Ny-Smira gene, can be recommended for selection of PVY-resistant progeny of cv. Sarpo Mira.
- Subjects :
- Hypersensitive response
Genetics
biology
fungi
Resistance
Potyvirus
food and beverages
Locus (genetics)
Plant Science
Marker
biology.organism_classification
Petunia
Article
PVY
MAS
Potato virus Y
Mapping
Genetic marker
Plant virus
Agronomy and Crop Science
Molecular Biology
Solanaceae
Biotechnology
Solanum tuberosum
Subjects
Details
- Language :
- English
- ISSN :
- 15729788 and 13803743
- Volume :
- 34
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Molecular Breeding
- Accession number :
- edsair.doi.dedup.....7b5fb379a29dc8de505ca1d2c94579ff