Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide for bone regeneration

Made available in DSpace on 2018-12-11T17:32:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-10-01 Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome. Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide [OGP(10-14)] for bone regeneration purposes. The BC-COL nanocomposites were successfully obtained by carbodiimide-mediated coupling as demonstrated by spectroscopy analysis. SEM, FTIR and 31P NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite. The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype. Additionally, the association among collagen, apatite, and OGP peptides enhanced cell growth compared with OGP-containing BC-Ap. Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects. These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications. São Paulo State University UNESP Institute of Chemistry Cell Culture Laboratory Faculty of Dentistry of Ribeirão Preto University of São Paulo USP São Paulo State University UNESP School of Dentistry at Araraquara Department of Genetics Faculty of Medicine of Ribeirão Preto University of São Paulo USP São Paulo State University UNESP Institute of Chemistry São Paulo State University UNESP School of Dentistry at Araraquara