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Runx2 stimulates neoangiogenesis through the Runt domain in melanoma
- Source :
- Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019), Scientific Reports
- Publication Year :
- 2019
-
Abstract
- Runx2 is a transcription factor involved in melanoma cell migration and proliferation. Here, we extended the analysis of Runt domain of Runx2 in melanoma cells to deepen understanding of the underlying mechanisms. By the CRISPR/Cas9 system we generated the Runt KO melanoma cells 3G8. Interestingly, the proteome analysis showed a specific protein signature of 3G8 cells related to apoptosis and migration, and pointed out the involvement of Runt domain in the neoangiogenesis process. Among the proteins implicated in angiogenesis we identified fatty acid synthase, chloride intracellular channel protein-4, heat shock protein beta-1, Rho guanine nucleotide exchange factor 1, D-3-phosphoglycerate dehydrogenase, myosin-1c and caveolin-1. Upon querying the TCGA provisional database for melanoma, the genes related to these proteins were found altered in 51.36% of total patients. In addition, VEGF gene expression was reduced in 3G8 as compared to A375 cells; and HUVEC co-cultured with 3G8 cells expressed lower levels of CD105 and CD31 neoangiogenetic markers. Furthermore, the tube formation assay revealed down-regulation of capillary-like structures in HUVEC co-cultured with 3G8 in comparison to those with A375 cells. These findings provide new insight into Runx2 molecular details which can be crucial to possibly propose it as an oncotarget of melanoma.
- Subjects :
- Proteomics
Vascular Endothelial Growth Factor A
0301 basic medicine
Skin Neoplasms
Angiogenesis
Datasets as Topic
lcsh:Medicine
Apoptosis
Core Binding Factor Alpha 1 Subunit
0302 clinical medicine
Cell Movement
Runx2
lcsh:Science
Tube formation
Multidisciplinary
Neovascularization, Pathologic
Chemistry
Runt
Endoglin
Runx2: Runt domain
Cell migration
Publisher Correction
Cell biology
Protein-protein interaction networks
Gene Expression Regulation, Neoplastic
Platelet Endothelial Cell Adhesion Molecule-1
RUNX2
Melanocytes
Guanine nucleotide exchange factor
neoangiogenesis
Primary Cell Culture
Article
03 medical and health sciences
Protein Domains
Cell Line, Tumor
Heat shock protein
Runx2, Runt, melanoma, neoangiogenesis
Biomarkers, Tumor
Human Umbilical Vein Endothelial Cells
melanoma
Humans
Melanoma
Protein–protein interaction networks
Transcription factor
lcsh:R
Computational Biology
Coculture Techniques
030104 developmental biology
lcsh:Q
CRISPR-Cas Systems
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019), Scientific Reports
- Accession number :
- edsair.doi.dedup.....7b49772e82189545b117db0e746b8f3e