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Improved immunogenicity of Newcastle disease virus inactivated vaccine following DNA vaccination using Newcastle disease virus hemagglutinin-neuraminidase and fusion protein genes
- Source :
- Journal of Veterinary Science
- Publication Year :
- 2016
- Publisher :
- The Korean Society of Veterinary Science, 2016.
-
Abstract
- The present study describes the development of DNA vaccines using the hemagglutinin-neuraminidase (HN) and fusion (F) genes from AF2240 Newcastle disease virus strain, namely pIRES/HN, pIRES/F and pIRES-F/HN. Transient expression analysis of the constructs in Vero cells revealed the successful expression of gene inserts in vitro. Moreover, in vivo experiments showed that single vaccination with the constructed plasmid DNA (pDNA) followed by a boost with inactivated vaccine induced a significant difference in enzyme-linked immunosorbent assay antibody levels (p < 0.05) elicited by either pIRES/F, pIRES/F+ pIRES/HN or pIRES-F/HN at one week after the booster in specific pathogen free chickens when compared with the inactivated vaccine alone. Taken together, these results indicated that recombinant pDNA could be used to increase the efficacy of the inactivated vaccine immunization procedure.
- Subjects :
- 0301 basic medicine
DNA vaccine
Newcastle Disease
Newcastle disease virus
Antibodies, Viral
Newcastle disease
Virus
DNA vaccination
03 medical and health sciences
Immunogenicity, Vaccine
Chlorocebus aethiops
Vaccines, DNA
Animals
HN Protein
Vero Cells
General Veterinary
biology
Viral Vaccine
Viral Vaccines
antibody response
inactivated vaccine
biology.organism_classification
Virology
Molecular biology
Specific Pathogen-Free Organisms
Vaccination
030104 developmental biology
Vaccines, Inactivated
Inactivated vaccine
Original Article
Hemagglutinin-neuraminidase
Chickens
Viral Fusion Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 1976555X and 1229845X
- Volume :
- 17
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Journal of Veterinary Science
- Accession number :
- edsair.doi.dedup.....7b46a376d99210fd050360f93452a355